Molecular properties of apelin: tissue distribution and receptor binding
Molecular properties of apelin: tissue distribution and receptor binding
复制标题
DOI：
10.1016/s0167-4889(00)00143-9
复制
发表时间：
2001-04-23
影响因子：
5.1
通讯作者：
Fujino, M
中科院分区：
文献类型：
--
作者：
Kawamata, Y;Fukusumi, S;Fujino, M
我们通过定量逆转录-聚合酶链反应分析了大鼠apelin mRNA的组织分布，并通过使用单克隆抗体的酶免疫测定法（EIA）分析了免疫反应性apelin（IR-apelin）的组织分布。apelin mRNA和ir-apelin的表达水平在组织间似乎是一致的：它们在肺和乳腺中高度表达。通过凝胶过滤和EIA的组合，nle发现apelin的分子形式在各个组织中不同：大小接近apelin-36（长型）的apelin分子是肺、睾丸和子宫中的主要组分，但在乳腺中检测到长型和短型（其大小接近[Glu（65）]apelin-13）。在Scatchard分析中，放射性碘标记的爱帕琳-36类似物与受体结合。APJ，具有高亲和力。在竞争性结合测定中，爱帕琳-36和爱帕琳-19比[Glu（65）]爱帕琳-13更有效地抑制标记的爱帕琳-36类似物与APJ的结合。在爱帕琳从APJ解离的分析中，未标记的爱帕琳-36比[Glu（65）]爱帕琳-13更快地取代与APJ结合的标记的爱帕琳-36类似物。我们的研究结果表明，长和短形式的爱帕琳不同地与APJ相互作用。 Elsevier Science B. V.保留所有权利。
We analyzed the tissue distribution of apelin mRNA in rats by a quantitative revere transcription-polymerase chain reaction and that of immunoreactive apelin (ir-apelin) by an enzyme immunoassay (EIA) using a monoclonal antibody. The expression levels of apelin mRNA and ir-apelin seemed to be consistent among tissues: they were highly expressed in the lung and mammary gland. By the combination of gel filtration and EIA, nle found that the molecular forms of apelin differ among respective tissues: apelin molecules with sizes close to apelin-36 (long forms) were major components ill the lung, testis, and uterus, but both long and short (whose sizes were close to [< Glu(65)]apelin-13) forms were detected in the mammary gland. In Scatchard analyses, the radioiodinated apelin-36 analogue bound to the receptor. APJ, with high affinity. In competitive binding assays, apelin-36 and apelin-19 far more efficiently inhibited the binding of the labeled apelin-36 analogue with APJ than [< Glu(65)]apelin-13. In analyses for the dissociation of apelin from APJ, unlabeled apelin-36 replaced mole rapidly the labeled apelin-36 analogue bound with APJ than [ < Glu(65)]apelin-13. Our results demonstrate that the long and short forms of apelin differently interact with APJ. Elsevier Science B.V. All rights reserved.