INTERNALIZATION OF LECTINS IN NEURONAL GERL
INTERNALIZATION OF LECTINS IN NEURONAL GERL
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DOI：
10.1083/jcb.73.1.1
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发表时间：
1977-01-01
影响因子：
7.8
通讯作者：
AVRAMEAS, S
中科院分区：
文献类型：
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作者：
GONATAS, NK;KIM, SU;AVRAMEAS, S
用蓖麻毒素凝集素和植物血凝素与辣根过氧化物酶(HRP)的结合物，对培养的小鼠背根神经节神经元的质膜受体内化进行了细胞化学研究。用凝集素-辣根过氧化物酶在4度进行细胞标记。C，37度内化。C在不含凝集素-HRP的培养液中，37℃孵育15-30分钟。凝集素-HRP受体复合体存在于靠近质膜的小泡或小管中。在37℃下放置1-3小时。在高尔基体的反面，凝集素-HRP受体复合体聚集在与富含酸性磷酸酶的小泡和小管(GERL)对应的小泡和小管中。37℃孵育3~6h后，少数包被的囊泡和可能的致密小体内含有HRP。C.可溶性HRP在本实验条件下或在37℃孵育时不被内吞。线粒体呼吸抑制剂可减少或抑制凝集素-HRP-受体结合物的内化，而细胞松弛素B或秋水仙素则不能。凝集素标记的神经细胞质膜部分明显主要内吞于GERL分子中。
Conjugates of ricin agglutinin and phytohemagglutinin with horseradish peroxidase (HRP) were used for a cytochemical study of internalization of their plasma membrane receptors in cultured isolated mouse dorsal root ganglion neurons. Labeling of cells with lectin-HRP was done at 4.degree. C, and internalization was performed at 37.degree. C in a culture medium free of lectin-HRP; 15-30 min after incubation at 37.degree. C, lectin-HRP-receptor complexes were seen in vesicles or tubules located near the plasma membrane. After 1-3 h at 37.degree. C, lectin-HRP-receptor complexes accumulated in vesicles and tubules corresponding to acid phosphatase-rich vesicles and tubules (GERL) at the trans aspect of the Golgi apparatus. A few coated vesicles and probably some dense bodies contained HRP after 3-6 h of incubation at 37.degree. C. Soluble HRP was not endocytosed under the conditions of this experiment or when it was present in the incubation medium at 37.degree. C. Internalization of lectin-HRP-receptor conjugates was decreased or inhibited by mitochondrial respiration inhibitors but not by cytochalasin B or colchicine. Lectin-labeled plasma membrane moieties of neurons are apparently endocytosed primarily in elements of GERL.