TREPONEMA-SUCCINIFACIENS SP-NOV, AN ANAEROBIC SPIROCHETE FROM THE SWINE INTESTINE
TREPONEMA-SUCCINIFACIENS SP-NOV, AN ANAEROBIC SPIROCHETE FROM THE SWINE INTESTINE
复制标题
DOI：
10.1007/bf00411285
复制
发表时间：
1979-01-01
影响因子：
2.8
通讯作者：
CANALEPAROLA, E
中科院分区：
文献类型：
--
作者：
CWYK, WM;CANALEPAROLA, E
对分离自猪结肠的一株专性厌氧螺旋体的形态、一般生理特性和产能代谢进行了研究。电镜观察表明，螺旋螺旋体细胞有一个外鞘、一个原生质柱和4个2-4-2排列的周质原纤维。螺旋体在含有碳水化合物、NaHCO3、瘤胃液、酵母膏、蛋白胨、L-半胱氨酸和无机盐的预还原液中于氮气气氛中生长。螺旋体发酵碳水化合物和生长所需的底物量二氧化碳(HCO3-)。氨基酸未发酵。以葡萄糖为底物，在CO2存在下生长的细胞发酵产物主要有醋酸盐、甲酸盐、琥珀酸和乳酸。还生成了少量的2，3-丁二醇、丙酮酸和乙酸乙酯。[1-14C]葡萄糖细胞抽提物中的酶活性和生长细胞产物中的放射性测定表明，该糖通过Embden-Meyerhof途径异化为丙酮酸。螺旋体利用一种类似于大肠菌群的碎裂反应来代谢丙酮酸。由[14C]NaHCO3形成的产物的放射性测定表明，二氧化碳被同化并用于生产丁二酸酯。DNA中鸟嘌呤+胞嘧啶的含量为36mol%。本研究表明，该肠道螺旋体是一种新的密螺旋体。建议将该新种命名为T.Sucinifaciens。
The morphology, the general physiological characteristics and the energy-yielding metabolism of an obligately anaerobic spirochete isolated from the colon of a swine were studied. EM showed that the helical spirochetal cells possessed an outer sheath, a protoplasmic cylinder and 4 periplasmic fibrils in a 2-4-2 arrangement. The spirochete grew in an atmosphere of N2 in prereduced media containing a carbohydrate, NaHCO3, rumen fluid, yeast extract, peptone, L-cysteine and inorganic salts. The spirochete fermented carbohydrates and required substrate amounts of CO2 (HCO3-) for growth. Amino acids were not fermented. Major fermentation products of cells growing with glucose as the substrate and in the presence of CO2 were acetate, formate, succinate and lactate. Small amounts of 2,3-butanediol, pyruvate and acetoin were also formed. Determinations of enzymatic activities in cell extracts and of radioactivity in products formed by growing cells from [1-14C]glucose indicated that this sugar was dissimilated to pyruvate via the Embden-Meyerhof pathway. The spirochetes used a coliform-type clastic reaction to metabolize pyruvate. Determinations of radioactivity in products formed from [14C]NaHCO3 indicated that CO2 was assimilated and used in succinate production. The guanine+cytosine content of the DNA was 36 mol%. This study indicates that this intestinal spirochete represents a new Treponema sp. It is proposed that the new species be named T. succinifaciens.