Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles
Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles
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DOI：
10.1128/aem.00407-11
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Gantzer, C.
中科院分区：
文献类型：
--
作者：
Dika, C.;Duval, J. F. L.;Gantzer, C.
我们首次比较了MS2噬菌体（pH为2.5 ~ 7，NaNO3电解质浓度为1 ~ 100 mM）与完全缺乏内部病毒RNA成分的相应病毒样颗粒（VLPs）的电动力学和聚集特性。与我们之前的工作（J. Langlet， F. Gaboriaud， C. Gantzer，和J. F. L. Duval，《生物物理学》）一致。J. 94:3293-3312, 2008)，发现修改病毒内RNA的内容导致MS2和MS2 VLPs的电流体动力学和聚集谱非常不同。在一定的pH和浓度条件下，MS2 VLPs的电泳迁移率大于MS2，且两者具有相似的等电点（IEP）值（接近4）。电动力学结果反映了MS2 VLPs对电渗透流的更大渗透性，在这些软颗粒在外加电场作用下迁移过程中，在其内部或周围形成了电渗透流。结果还支持在VLPs中存在一些剩余的负电荷成分。此外，无论溶液离子强度的大小，MS2噬菌体在低于IEP的pH值下系统地形成聚集体，而MS2 VLPs在足够低的盐浓度下，在pH相对等于IEP的严格条件下聚集(
We compare for the first time the electrokinetic and aggregation properties of MS2 phage (pH 2.5 to 7, 1 to 100 mM NaNO3 electrolyte concentration) with those of the corresponding virus-like particles (VLPs), which lack entirely the inner viral RNA component. In line with our previous work (J. Langlet, F. Gaboriaud, C. Gantzer, and J. F. L. Duval, Biophys. J. 94:3293-3312, 2008), it is found that modifying the content of RNA within the virus leads to very distinct electrohydrodynamic and aggregation profiles for MS2 and MS2 VLPs. Under the given pH and concentration conditions, MS2 VLPs exhibit electrophoretic mobility larger in magnitude than that of MS2, and both have similar isoelectric point (IEP) values (similar to 4). The electrokinetic results reflect a greater permeability of MS2 VLPs to electroosmotic flow, developed within/around these soft particles during their migration under the action of the applied electrical field. Results also support the presence of some remaining negatively charged component within the VLPs. In addition, MS2 phage systematically forms aggregates at pH values below the IEP, regardless of the magnitude of the solution ionic strength, whereas MS2 VLPs aggregate under the strict condition where the pH is relatively equal to the IEP at sufficiently low salt concentrations (