Bactericidal efficacy of nitric oxide-releasing silica nanoparticles
Bactericidal efficacy of nitric oxide-releasing silica nanoparticles
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DOI：
10.1021/nn700191f
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发表时间：
2008-02-01
期刊：
影响因子：
17.1
通讯作者：
Schoenfisch, Mark H.
中科院分区：
文献类型：
--
作者：
Hetrick, Evan M.;Shin, Jae Ho;Schoenfisch, Mark H.
一氧化氮（NO）释放二氧化硅纳米颗粒作为新型抗菌药物的效用证明了铜绿假单胞菌。通过四烷氧基硅烷与氨基烷氧基硅烷的缩聚，制备了一氧化氮释放纳米颗粒，使其能够储存大量的一氧化氮有效载荷。将释放NO的纳米颗粒与小分子NO供体1-[2-（羧基）吡咯烷-1-酰基]重氮-1-ium-1,2- diazen-1-ium-1,2-diolate （PROLI/NO）的杀菌效果进行比较，发现纳米颗粒衍生的NO的杀菌效果增强，并降低了对健康细胞（哺乳动物成纤维细胞）的细胞毒性。共聚焦显微镜显示，荧光标记的no释放纳米颗粒与细菌细胞相关，为纳米颗粒增强的杀菌效果提供了基本原理。当NO由纳米颗粒递送时，细胞内NO浓度可测量，而不是PROLI/NO。总的来说，这些结果证明了通过纳米颗粒输送NO用于抗菌应用的优势。
The utility of nitric oxide (NO)-releasing silica nanoparticles as novel antibacterial agents is demonstrated against Pseudomonas aeruginosa. Nitric oxide-releasing nanoparticles were prepared via cocondensation of tetraalkoxysilane with aminoalkoxysilane modified with diazeniumdiolate NO donors, allowing for the storage of large NO payloads. Comparison of the bactericidal efficacy of the NO-releasing nanoparticles to 1-[2-(carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolate (PROLI/NO), a small molecule NO donor, demonstrated enhanced bactericidal efficacy of nanoparticle-derived NO and reduced cytotoxicity to healthy cells (mammalian fibroblasts). Confocal microscopy revealed that fluorescently labeled NO-releasing nanoparticles associated with the bacterial cells, providing rationale for the enhanced bactericidal efficacy of the nanoparticles. Intracellular NO concentrations were measurable when the NO was delivered from nanoparticles as opposed to PROLI/NO. Collectively, these results demonstrate the advantage of delivering NO via nanoparticles for antimicrobial applications.