Complement-Dependent Lysis of Influenza A Virus-Infected Cells by Broadly Cross-Reactive Human Monoclonal Antibodies
Complement-Dependent Lysis of Influenza A Virus-Infected Cells by Broadly Cross-Reactive Human Monoclonal Antibodies
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DOI：
10.1128/jvi.05193-11
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发表时间：
2011-12-01
影响因子：
5.4
通讯作者：
Ennis, Francis A.
中科院分区：
文献类型：
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作者：
Terajima, Masanori;Cruz, John;Ennis, Francis A.
我们通过补体依赖性裂解（CDL）试验鉴定了从流感病毒感染患者和流感疫苗接种者中克隆的人单克隆抗体（MAbs）。CDL中的大多数单克隆抗体是中和性的，但不是所有的中和性单克隆抗体都能介导CDL。测试的三种茎特异性中和单克隆抗体中的两种能够介导CDL，并且与时间上遥远的H1N1毒株的交叉反应性比常规的血凝抑制和中和单克隆抗体更强。其中一种茎特异性单克隆抗体与H1和H2血凝素具有亚型交叉反应性，表明茎特异性抗体在预防流感疾病中的作用，特别是通过一种可引起大流行的新型病毒亚型。
We characterized human monoclonal antibodies (MAbs) cloned from influenza virus-infected patients and from influenza vaccine recipients by complement-dependent lysis (CDL) assay. Most MAbs active in CDL were neutralizing, but not all neutralizing MAbs can mediate CDL. Two of the three stalk-specific neutralizing MAbs tested were able to mediate CDL and were more cross-reactive to temporally distant H1N1 strains than the conventional hemagglutination-inhibiting and neutralizing MAbs. One of the stalk-specific MAbs was subtype cross-reactive to H1 and H2 hemagglutinins, suggesting a role for stalk-specific antibodies in protection against influenza illness, especially by a novel viral subtype which can cause pandemics.