Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial-related genes in longissimus dorsi muscle and liver of piglets
Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial-related genes in longissimus dorsi muscle and liver of piglets
复制标题
日粮亮氨酸对仔猪背最长肌和肝脏抗氧化活性及抗氧化和线粒体相关基因表达的影响
DOI：
10.1111/asj.13249
复制
发表时间：
2019-06-28
影响因子：
2
通讯作者：
Huang, Zhiqing
中科院分区：
文献类型：
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作者：
Chen, Xiaoling;Xiang, Lu;Huang, Zhiqing
本研究旨在探讨饲粮中添加亮氨酸对仔猪背最长肌和肝脏抗氧化活性及抗氧化相关基因表达的影响。三种日粮中添加不同水平的亮氨酸（0%，0.25%，0.5%）。结果表明，与对照组相比，添加0.25%亮氨酸显著提高了仔猪血清、背最长肌和肝脏中抗超氧阴离子（阿萨）和抗羟自由基（AHR）水平，以及总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）活性和总抗氧化能力（T-AOC）。添加0.25%亮氨酸显著提高了背最长肌和肝脏中SOD 2、CAT、GPx、GST、谷胱甘肽还原酶（GR）和核因子红细胞2相关因子2（Nrf 2）的mRNA水平。而丙二醛（MDA）含量和Kelch样ECH相关蛋白1（Keap 1）mRNA水平则呈相反趋势。此外，添加0.25%亮氨酸显著提高了仔猪背最长肌和肝脏中与哺乳相关基因的mRNA水平。结果表明，添加0.25%亮氨酸可提高仔猪的抗氧化活性和线粒体的生物合成与功能，其机制与提高抗氧化酶活性和上调抗氧化及线粒体相关基因的表达有关。
The study was conducted to investigate the effects of dietary leucine on antioxidant activity and expression of antioxidant- and mitochondrial-related genes in longissimus dorsi muscle and liver of piglets. Three diets were formulated with different levels of supplemented leucine (0%, 0.25%, 0.5%). Results showed that supplementation of 0.25% leucine significantly increased antisuperoxide anion (ASA) and antihydroxyl radical (AHR) levels and activities of total superoxide dismutade (T-SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total antioxidant capacity (T-AOC) in serum, longissimus dorsi muscle and liver of piglets as compared with the control group. The SOD2, catalase (CAT), GPx, GST, glutathione reductase (GR), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels in longissimus dorsi muscle and liver were significantly increased by 0.25% leucine supplementation. However, the malondialdehyde (MDA) content and the mRNA level of Kelch-like ECH-associated protein 1 (Keap1) exhibited an opposite tendency. Additionally, supplementation of 0.25% leucine significantly increased the mRNA levels of mitochondrial-related genes in longissimus dorsi muscle and liver of piglets. Results suggested that supplementation of 0.25% leucine improved antioxidant activity and mitochondrial biogenesis and function of piglets, which was related to the increase in antioxidant enzymes activities and upregulation of expression of antioxidant- and mitochondrial-related genes.