Comparative sequence analysis as a tool for studying the secondary structure of mRNAs.
Comparative sequence analysis as a tool for studying the secondary structure of mRNAs.
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比较序列分析作为研究 mRNA 二级结构的工具。
DOI：
10.1093/nar/13.23.8645
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发表时间：
1985
影响因子：
14.9
通讯作者：
Lawrence,CB
中科院分区：
文献类型：
--
作者：
Browner,MF;Lawrence,CB
系统发育保守二级结构的分析对于结构 RNA 二级结构模型的开发非常重要。在本文中，我们将这种类型的分析应用于几个信息 RNA 家族，以评估其在开发 mRNA 和 mRNA 前体二级结构模型中的有用性。我们在所有分析的 mRNA 组中观察到许多保守螺旋。使用三个标准来鉴定潜在的螺旋，这些螺旋不仅仅由于编码序列的限制而保守，因此可能对 RNA 的结构和功能很重要。这些结果表明，当与其他互补技术结合使用时，这种方法将有助于推导信息 RNA 的二级结构模型，以及设计实验以确定保守碱基配对相互作用的功能意义。
Analysis of phylogenetically conserved secondary structure has been important in the development of models for the secondary structure of structural RNAs. In this paper, we apply this type of analysis to several families of informational RNAs to evaluate its usefulness in developing secondary structure models for mRNAs and mRNA precursors. We observed many conserved helices in all mRNA groups analyzed. Three criteria were used to identify potential helices which were not conserved solely because of coding sequence constraints, and may therefore be important for the structure and function of the RNA. These results suggest that this approach will be useful in deriving secondary structure models for informational RNAs when used in conjunction with other complementary techniques, and in designing experiments to determine the functional significance of conserved base pairing interactions.