OsGLU3, a putative membrane-bound endo-1,4-beta-glucanase, is required for root cell elongation and division in rice (Oryza sativa L.).
OsGLU3, a putative membrane-bound endo-1,4-beta-glucanase, is required for root cell elongation and division in rice (Oryza sativa L.).
复制标题
DOI：
10.1093/mp/ssr084
复制
发表时间：
2012
期刊：
影响因子：
27.5
通讯作者：
Jinwei Zhang;Lei Xu;Yunrong Wu;Xinai Chen;Yu Liu;Shihua Zhu;W. Ding;Ping Wu;K. Yi
中科院分区：
文献类型：
--
作者：
Jinwei Zhang;Lei Xu;Yunrong Wu;Xinai Chen;Yu Liu;Shihua Zhu;W. Ding;Ping Wu;K. Yi
植物的根通过伸长在土壤中移动。这对它们锚住植物并从土壤中获取水和矿物质的能力至关重要。为了鉴定水稻根伸长相关的新基因，我们从甲基磺酸乙酯（EMS）诱变水稻文库中筛选出一个短根突变体Osglu 3 -1。图位克隆结果表明，该突变体是由于OsGLU 3基因的一个点突变引起的，OsGLU 3基因编码一种膜结合内切1，4-β-葡聚糖酶。OsGlu 3 -1的根细胞壁中结晶纤维素含量较少，根细胞长度较短，根分生组织略小，这通过OsCYCB 1，1：GUS的限制性表达来观察。外源葡萄糖处理可抑制Osglu 3 -1的低根细胞壁纤维素含量和短根表型。OsGLU 3在植物的各种组织中都有广泛表达，在根尖、侧根和冠根原基中表达量较高。在质膜中检测到完全功能的OsGLU 3-GFP，并且在根分生组织和伸长区中检测到FM 4 -64标记的隔室。我们还发现，磷酸盐饥饿，环境胁迫，改变细胞壁纤维素含量调节根伸长在OsGLU 3依赖的方式。
Plant roots move through the soil by elongation. This is vital to their ability to anchor the plant and acquire water and minerals from the soil. In order to identify new genes involved in root elongation in rice, we screened an ethyl methane sulfonate (EMS)-mutagenized rice library, and isolated a short root mutant, Osglu3-1. The map-based cloning results showed that the mutant was due to a point mutation in OsGLU3, which encodes a putative membrane-bound endo-1,4-β-glucanase. Osglu3-1 displayed less crystalline cellulose content in its root cell wall, shorter root cell length, and a slightly smaller root meristem as visualized by restricted expression of OsCYCB1,1:GUS. Exogenous application of glucose can suppress both the lower root cell wall cellulose content and short root phenotypes of Osglu3-1. Consistently, OsGLU3 is ubiquitously expressed in various tissues with strong expression in root tip, lateral root, and crown root primodia. The fully functional OsGLU3-GFP was detected in plasma membrane, and FM4-64-labeled compartments in the root meristem and elongation zones. We also found that phosphate starvation, an environmental stress, altered cell wall cellulose content to modulate root elongation in a OsGLU3-dependant way.