RNF114 Silencing Inhibits the Proliferation and Metastasis of Gastric Cancer.
RNF114 Silencing Inhibits the Proliferation and Metastasis of Gastric Cancer.
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RNF114沉默抑制胃癌的增殖和转移。
DOI：
10.7150/jca.62033
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发表时间：
2022
影响因子：
3.9
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Feng Z;Li L;Zeng Q;Zhang Y;Tu Y;Chen W;Shu X;Wu A;Xiong J;Cao Y;Li Z
RNF 114（E3泛素连接酶RING指蛋白114）首先被鉴定为促进银屑病发展的锌结合蛋白;然而，其在胃癌中的作用仍不清楚。本研究运用生物信息学和分子生物学技术探讨RNF 114与胃癌的关系。结果显示，RNF 114在胃癌组织中呈高表达，且与患者预后呈负相关。功能分析表明RNF 114沉默在一定程度上抑制了胃癌细胞的增殖和转移。进一步的研究表明，RNF 114表达可能被miR-218- 5 p和甲基化修饰靶向，并通过泛素化途径介导下游EGR 1（早期生长反应1）降解。总之，目前的结果突出了RNF 114过表达在胃癌中的不利影响，并有助于更好地理解RNF 114功能的机制。
RNF114 (E3 ubiquitin ligase RING finger protein 114) was first identified as a zinc-binding protein that promotes psoriasis development; however, its role in gastric cancer is still unclear. We explored the relationship between RNF114 and gastric cancer using bioinformatics and molecular biology techniques. The results showed that RNF114 was highly expressed in gastric cancer and negatively correlated with the patient's prognosis. Functional assays suggested that RNF114 silencing suppressed the proliferation and metastasis of gastric cancer cells to a certain extent. Further studies showed that RNF114 expression was potentially targeted by miR-218-5p and methylation modification, and mediated downstream EGR1 (early growth response 1) degradation by the ubiquitylation approach. Together, the present results highlight the detrimental effects of RNF114 overexpression in gastric cancer and contribute to a better understanding of the mechanisms underlying RNF114 functionality.