A cost-effective and field-ready potentiostat that poises subsurface electrodes to monitor bacterial respiration
A cost-effective and field-ready potentiostat that poises subsurface electrodes to monitor bacterial respiration
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DOI：
10.1016/j.bios.2011.12.013
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发表时间：
2012-02-15
影响因子：
12.6
通讯作者：
Angenent, Largus T.
中科院分区：
文献类型：
--
作者：
Friedman, Elliot S.;Rosenbaum, Miriam A.;Angenent, Largus T.
在这里，我们提出了基于地下生物电化学系统（BES）的生物传感器的概念验证，该传感器能够监测通过细胞外电子转移发生的微生物呼吸。该系统包括我们基于三通道微控制器单元 (MCU) 的恒电位仪的开源设计，该恒电位仪能够进行计时电流分析，实验室测试表明其准确度在商业恒电位仪的 0.95 +/- 0.58%（95% 置信限）内。恒电位仪设计可在线免费获取：http://angenentbee.cornel.edu/potiostat.html。这种坚固耐用且可现场使用的恒电位仪可以承受 -30 摄氏度的温度，制造成本相对较低（600 美元），因此可以在现场进行大规模部署。基于 MCU 的恒电位仪与电极和基于太阳能电池板的电源系统集成，并部署为生物传感器，用于监测阿拉斯加州巴罗郊外的排干解冻湖盆地中的微生物呼吸。在三个不同的深度，微生物三电极系统（M3C）的工作电极保持在与铁（III）化合物和腐殖酸的微生物还原相对应的电位。因此，工作电极模拟这些化合物并被某些微生物用作电子受体。传感器揭示了微生物呼吸的日常周期。在中深度和深层电极中，这些循环的开始伴随着总体活动的显着增加，这对应于随着夏季解冻的进行，这些土壤达到有利于微生物活动的温度。 BES 生物传感器是研究偏远环境中微生物活动的重要工具，恒电位仪的经济高效设计允许在偏远地区广泛使用。 (C) 2011 Elsevier B.V. 保留所有权利。
Here, we present the proof-of-concept for a subsurface bioelectrochemical system (BES)-based biosensor capable of monitoring microbial respiration that occurs through exocellular electron transfer. This system includes our open-source design of a three-channel microcontroller-unit (MCU)-based potentiostat that is capable of chronoamperometry, which laboratory tests showed to be accurate within 0.95 +/- 0.58% (95% Confidence Limit) of a commercial potentiostat. The potentiostat design is freely available online: http://angenentbee.cornel.edu/potentiostat.html. This robust and field-ready potentiostat, which can withstand temperatures of -30 degrees C, can be manufactured at relatively low cost ($600), thus, allowing for en-masse deployment at field sites. The MCU-based potentiostat was integrated with electrodes and a solar panel-based power system, and deployed as a biosensor to monitor microbial respiration in drained thaw lake basins outside Barrow, AK. At three different depths, the working electrode of a microbial three-electrode system (M3C) was maintained at potentials corresponding to the microbial reduction of iron(III) compounds and humic acids. Thereby, the working electrode mimics these compounds and is used by certain microbes as an electron acceptor. The sensors revealed daily cycles in microbial respiration. In the medium- and deep-depth electrodes the onset of these cycles followed a considerable increase in overall activity that corresponded to those soils reaching temperatures conducive to microbial activity as the summer thaw progressed. The BES biosensor is a valuable tool for studying microbial activity in situ in remote environments, and the cost-efficient design of the potentiostat allows for wide-scale use in remote areas. (C) 2011 Elsevier B.V. All rights reserved.