Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies.
Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies.
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DOI：
10.1002/anie.200605237
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发表时间：
2007-04
影响因子：
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通讯作者：
Peter S. C. Wu;K. Ozawa;S. Lim;S. Vasudevan;N. Dixon;G. Otting
中科院分区：
文献类型：
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作者：
Peter S. C. Wu;K. Ozawa;S. Lim;S. Vasudevan;N. Dixon;G. Otting
快速和干净：在无细胞蛋白质合成中，通过使用聚合酶链式反应（PCR）扩增的DNA模板可以获得优异的蛋白质产率，条件是模板被设计用于环化。这消除了耗时的克隆步骤，并允许在24小时内从互补DNA到蛋白质NMR谱。（图为介绍）。
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h.(Graph Presented).