AN ATPE-SPECIFIC PROMOTER WITHIN THE CODING REGION OF THE ATPB GENE IN TOBACCO CHLOROPLAST DNA
AN ATPE-SPECIFIC PROMOTER WITHIN THE CODING REGION OF THE ATPB GENE IN TOBACCO CHLOROPLAST DNA
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DOI：
10.1007/bf00309558
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发表时间：
1994-09-01
期刊：
影响因子：
2.5
通讯作者：
SUGIURA, M
中科院分区：
文献类型：
--
作者：
KAPOOR, S;WAKASUGI, T;SUGIURA, M
atpB和atpE基因分别编码叶绿体ATP合酶的β和β亚基，并且在迄今为止所研究的植物物种中共转录。在烟草中，atpB基因特异性探针与2.7-和2.3-kb转录物杂交。除此之外，来自atpE编码区的探针也与1.0-kb转录物杂交。atpE特异性转录物的5'端定位于atpB基因编码区内atpE翻译起始位点上游430/431 nt处。体外加帽揭示了该转录物由初级转录事件产生，并且还以5'区域中的-10和-35典型序列为特征。已发现其与双顺反子转录物共有一个共同的3'端，该转录物已被定位在趋异转录的trnM基因的编码区内，在atpE终止密码子下游约236 nt处。有趣的是，这种转录物仅在叶中积累，而不在含前质体的培养细胞（BY-2）中积累，表明。除非它在BY-2细胞中优先降解，否则它的表达可能是转录控制的。
The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to a 1.0-kb transcript. The 5' end of the atpE-specific transcript has been mapped 430/431 nt upstream of the atpE translation initiation site, within the coding region of the atpB gene. In-vitro capping revealed that this transcript results from a primary transcriptional event and is also characterized by -10 and -35 canonical sequences in the 5' region. It has been found to share a common 3' end with the bi-cistronic transcripts that has been mapped within the coding region of the divergently transcribed trnM gene, approximately 236 nt downstream from the atpE termination codon. Interestingly, this transcript accumulates only in leaves and not in proplastid-containing cultured (BY-2) cells, indicating. that, unless it is preferentially degraded in BY-2 cells, its expression might be transcriptionally controlled.