Effects of two-dimensional materials on human mesenchymal stem cell behaviors.
Effects of two-dimensional materials on human mesenchymal stem cell behaviors.
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DOI：
10.1016/j.bbrc.2017.08.149
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发表时间：
2017-11
影响因子：
3.1
通讯作者：
Intan Rosalina Suhito;Yoojoong Han;Da-Seul Kim;H. Son;Tae-Hyung Kim
中科院分区：
文献类型：
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作者：
Intan Rosalina Suhito;Yoojoong Han;Da-Seul Kim;H. Son;Tae-Hyung Kim
石墨烯是一种典型的二维（2D）材料，已知其影响多种干细胞行为，包括粘附、铺展、生长和分化。在这里，我们首次报告了四种不同的新兴2D材料对人脂肪间充质干细胞（hADMSCs）的影响。选择氧化石墨烯（GO）、硫化钼（MoS 2）、硫化钨（WS 2）和氮化硼（BN）作为模型二维材料，并通过滴铸法将其涂覆在细胞培养基底上。在低浓度范围（<5 μg/ml）下，四种不同的2D材料中的任何一种都没有观察到急性毒性。有趣的是，与未处理的（NT）基底相比，2D材料改性的基底表现出更高的细胞粘附、扩散和增殖。值得注意的是，在分化的情况下，基于mRNA表达水平（qPCR）和脂滴的量（ORO染色），与NT和GO修饰的底物相比，MoS 2、WS 2和BN修饰的底物在引导hADMSC的脂肪形成方面表现出更好的性能。相比之下，发现在所有2D材料涂覆的基底中，GO涂覆的基底（50 μg/mL）最有效地诱导成骨。总之，2D材料可以作为控制干细胞生长和分化的有利来源，这在生物医学研究和治疗中可能非常有利。
Graphene, a typical two-dimensional (2D) material, is known to affect a variety of stem cell behaviors including adhesion, spreading, growth, and differentiation. Here, we report for the first time the effects of four different emerging 2D materials on human adipose-derived mesenchymal stem cells (hADMSCs). Graphene oxide (GO), molybdenum sulfide (MoS2), tungsten sulfide (WS2), and boron nitride (BN) were selected as model two-dimensional materials and were coated on cell-culture substrates by a drop-casting method. Acute toxicity was not observed with any of the four different 2D materials at a low concentration range (<5 μg/ml). Interestingly, the 2D material-modified substrates exhibited a higher cell adhesion, spreading, and proliferation when compared with a non-treated (NT) substrate. Remarkably, in the case of differentiation, the MoS2-, WS2-, and BN-modified substrates exhibited a better performance in terms of guiding the adipogenesis of hADMSCs when compared with both NT and GO-modified substrates, based on the mRNA expression level (qPCR) and amount of lipid droplets (ORO staining). In contrast, the osteogenesis was found to be most efficiently induced by the GO-coated substrate (50 μg/mL) among all 2D-material coated substrates. In summary, 2D materials could act as favorable sources for controlling the stem cell growth and differentiation, which might be highly advantageous in both biomedical research and therapy.