Cloning of the murine ER71 gene (Etsrp71) and initial characterization of its promoter
Cloning of the murine ER71 gene (Etsrp71) and initial characterization of its promoter
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DOI：
10.1016/j.ygeno.2004.12.003
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发表时间：
2005-04-01
期刊：
影响因子：
4.4
通讯作者：
Janknecht, R
中科院分区：
文献类型：
--
作者：
De Haro, L;Janknecht, R
ER71 蛋白属于 ETS 转录因子家族，在成年小鼠睾丸中特异性表达。在这里，我们描述了相应 Etsrp71 基因和启动子的克隆。鼠类Etsrp71基因相对紧凑，跨度为3 kb，排列成七个外显子和六个内含子，其中大部分在大鼠和人类中高度保守。其启动子没有 TATA 框，转录从多个位点开始。此外，存在两种 ER71 亚型，它们的 N 末端氨基酸有 22 个不同，但在 DNA 结合或反式激活方面没有差异。在转录起始位点附近，我们确定了转录因子 Sp1 的结合位点。该结合位点的突变严重削弱了 Sp1 激活 Etsrp71 启动子的能力。这里报告的发现可能为进一步研究阐明胚胎发生和成年睾丸中 Etsrp71 基因活性的调节提供途径。 (c) 2004 Elsevier Inc. 保留所有权利。
The ER71 protein belongs to the ETS transcription factor family and is testis-specifically expressed in adult mice. Here we describe the cloning of the respective Etsrp71 gene and promoter. The murine Etsrp71 gene is relatively compact, spanning 3 kb, and is arranged into seven exons and six introns, the majority of which are highly conserved in rat and human. Its promoter is devoid of a TATA box and transcription starts at Multiple sites. Furthermore, two ER71 isoforms exist that differ by 22 N-terminal amino acids, but show no difference in DNA binding or transactivation. Close to the transcription initiation sites, we identified a binding site for the transcription factor Sp1. Mutation of this binding site severely diminished the ability of Sp1 to activate the Etsrp71 promoter. The findings reported here may provide avenues for further research elucidating the regulation of Etsrp71 gene activity during embryogenesis and in adult testes. (c) 2004 Elsevier Inc. All rights reserved.