ROLE OF ORGANIC MATRIX IN URINARY STONE FORMATION - AN ULTRASTRUCTURAL-STUDY OF CRYSTAL MATRIX INTERFACE OF CALCIUM-OXALATE MONOHYDRATE STONES
ROLE OF ORGANIC MATRIX IN URINARY STONE FORMATION - AN ULTRASTRUCTURAL-STUDY OF CRYSTAL MATRIX INTERFACE OF CALCIUM-OXALATE MONOHYDRATE STONES
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DOI：
10.1016/s0022-5347(17)35454-x
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发表时间：
1993-07-01
影响因子：
6.6
通讯作者：
HACKETT, RL
中科院分区：
文献类型：
--
作者：
KHAN, SR;HACKETT, RL
用乙二胺四乙酸（EDTA）溶液和Karnovsky固定剂的混合物对人一水草酸钙（COM）尿结石进行脱钙处理，然后包埋在细菌琼脂中。通过光学显微镜、扫描和透射电子显微镜检查脱钙结石。结石有明显的核，被无定形或磷灰石磷酸钙或球晶COM晶体的聚集体占据。EDTA不溶性基质在结石中普遍存在，主要由精细缠结的纤维材料组成。它在石头的外围区域以同心叠层的形式组织起来，但在石头中心却显得非常混乱。水晶被水晶幽灵所取代。晶体内部和晶间空间均存在有机基质。研究表明，晶体和有机基质之间存在着非常密切的联系。这种关系似乎开始于晶体形成的早期，并在尿结石的形成和生长阶段持续存在。
Human calcium oxalate monohydrate (COM) urinary stones were decalcified by treatment with a mixture of ethylenediaminetetraacetic acid (EDTA) solution and Karnovsky's fixative after embedding in bactoagar. Decalcified stones were examined by light microscopy, and also by scanning and transmission electron microscopy. Stones had distinct nuclei that were occupied by amorphous or apatitic calcium phosphate or aggregates of spherulitic COM crystals. EDTA insoluble matrix was ubiquitous in stones and consisted largely of finely matted fibrous material. It was organized in concentric laminations in the peripheral area of the stone but appeared highly disorganized in the stone center. Crystals were replaced by crystal ghosts. Organic matrix was present both inside the crystals and in the intercrystalline spaces. The study indicates a very close association between crystals and organic matrix. The relationship appears to begin early in crystal formation and persists throughout the formative and growth phases of the urinary stones.