Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity: relationship with gut microbiome richness and ecology
Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity: relationship with gut microbiome richness and ecology
复制标题
DOI：
10.1136/gutjnl-2014-308778
复制
发表时间：
2016-03-01
期刊：
影响因子：
24.5
通讯作者：
Clement, Karine
中科院分区：
文献类型：
--
作者：
Dao, Maria Carlota;Everard, Amandine;Clement, Karine
目的肥胖和2型糖尿病患者的肠道微生物种类和微生物基因丰富度与瘦人和健康人不同。在小鼠中，黏液降解细菌嗜粘杆菌的丰度与体脂肪量和葡萄糖耐受性呈负相关，但在人类中需要更多的证据。饮食和减肥对这种细菌种类的影响尚不清楚。我们的目的是评估粪便嗜粘杆菌丰度、粪便微生物组基因丰富度、饮食、宿主特征及其热量限制（CR）后变化之间的关系。设计干预包括6周的CR期，随后是6周的体重稳定饮食，对象为超重和肥胖成人（N=49，包括41名女性）。在基线和CR和体重稳定后测量粪便嗜粘杆菌丰度、粪便微生物基因丰富度、饮食和生物临床参数。结果在基线时，嗜粘单胞杆菌与空腹血糖、腰臀比和皮下脂肪细胞直径呈负相关。基因丰富度和嗜粘杆菌丰度较高的受试者代谢状态最健康，尤其是在空腹血糖、血浆甘油三酯和体脂分布方面。在CR后，嗜粘杆菌基线较高的个体在胰岛素敏感性标记物和其他临床参数上表现出更大的改善，这些参与者也经历了嗜粘杆菌丰度的减少，但仍显著高于基线丰度较低的个体。嗜muciniphila与已知与健康相关的微生物物种有关。结论：嗜粘液芽胞杆菌与超重/肥胖成人CR后更健康的代谢状态和更好的临床结果相关。肠道菌群生态学与嗜粘单胞杆菌之间的相互作用值得进一步研究。
Objective Individuals with obesity and type 2 diabetes differ from lean and healthy individuals in their abundance of certain gut microbial species and microbial gene richness. Abundance of Akkermansia muciniphila, a mucin-degrading bacterium, has been inversely associated with body fat mass and glucose intolerance in mice, but more evidence is needed in humans. The impact of diet and weight loss on this bacterial species is unknown. Our objective was to evaluate the association between faecal A. muciniphila abundance, faecal microbiome gene richness, diet, host characteristics, and their changes after calorie restriction (CR).Design The intervention consisted of a 6-week CR period followed by a 6-week weight stabilisation diet in overweight and obese adults (N=49, including 41 women). Faecal A. muciniphila abundance, faecal microbial gene richness, diet and bioclinical parameters were measured at baseline and after CR and weight stabilisation.Results At baseline A. muciniphila was inversely related to fasting glucose, waist-to-hip ratio and subcutaneous adipocyte diameter. Subjects with higher gene richness and A. muciniphila abundance exhibited the healthiest metabolic status, particularly in fasting plasma glucose, plasma triglycerides and body fat distribution. Individuals with higher baseline A. muciniphila displayed greater improvement in insulin sensitivity markers and other clinical parameters after CR. These participants also experienced a reduction in A. muciniphila abundance, but it remained significantly higher than in individuals with lower baseline abundance. A. muciniphila was associated with microbial species known to be related to health.Conclusions A. muciniphila is associated with a healthier metabolic status and better clinical outcomes after CR in overweight/obese adults. The interaction between gut microbiota ecology and A. muciniphila warrants further investigation.