Immunity to Plasmodium berghei exoerythrocytic from irradiated sporozoites
Immunity to Plasmodium berghei exoerythrocytic from irradiated sporozoites
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DOI：
10.1007/s004360050117
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发表时间：
1996-04-01
影响因子：
2
通讯作者：
Wery, M
中科院分区：
文献类型：
--
作者：
Chatterjee, S;Francois, G;Wery, M
本文用辐射子孢子免疫的体内宿主保护参数和免疫小鼠血清抗体抑制子孢子侵入和伯氏疟原虫（Plasmodiumberghei）外红内期（EE）发育的体外参数，研究了辐射子孢子发育的EE期产生的免疫性质。在体内攻击免疫小鼠的子孢子，保护观察到的辐射剂量依赖性的方式。这一发现强调，保护作用取决于子孢子的辐照剂量，该剂量允许子孢子穿透但控制EE在肝脏内的形成。使用人肝癌细胞系Hep G2作为体外宿主细胞，我们观察到，在用辐射子孢子免疫的小鼠中产生的血清抗体在免疫荧光抗体试验（IFAT）中与子孢子和肝期寄生虫反应。未观察到与血液期寄生虫的反应性。用6至18 krad照射的子孢子免疫的小鼠的血清抗体抑制子孢子侵袭，并引起肝癌细胞中EE形式发展的严重抑制，指出从照射的子孢子（irra EE形式）发展的EE形式的抗原含量作为关键的免疫原。此外，在酶联免疫吸附试验（ELISA）中，对12-krad辐照的子孢子产生的血清抗体显示出对代表恶性疟原虫环子孢子（CS）蛋白的保守区域II序列以及基于恶性疟原虫肝脏阶段特异性抗原（LSA-1）的重复序列的合成肽的反应性，从而暗示子孢子和肝脏阶段在保护中的重要作用。
The nature of immunity generated by Plasmodium berghei exoerythrocytic (EE) stages developing from irradiated sporozoites was studied using in vivo parameters of host protection on immunization with irradiated sporozoites and in vitro parameters of inhibition of sporozoite invasion and EE form development by serum antibodies from immunized mice. On in vivo challenge of immunized mice by sporozoites, protection was observed in an irradiation-dose-dependent manner. This finding stresses that protection is dependent on the irradiation dose of sporozoites that allows sporozoite penetration yet controls EE form development within the liver. Using the human hepatoma line Hep G2 as host cells in vitro, we observed that serum antibodies raised in mice immunized with irradiated sporozoites reacted with sporozoite- and hepatic-stage parasites in an immunofluorescent antibody test (IFAT). No reactivity was observed with blood-stage parasites. Serum antibodies from mice immunized with 6- to 18-krad-irradiated sporozoites inhibited sporozoite invasion and caused severe inhibition of EE form development in hepatoma cells, pointing to the antigenic content of EE forms developing from irradiated sporozoites (irra EE forms) as critical immunogens. Moreover, in an enzyme-linked immunosorbent assay (ELISA), serum antibodies raised to 12-krad-irradiated sporozoites showed reactivity to synthetic peptides representing the conserved Region II sequences of the P. falciparum circumsporozoite (CS) protein as well as the P. falciparum liver-stage-specific antigen (LSA-1)-based repeat sequences, thus implicating an important role for both the sporozoite and the hepatic stage in protection.