Genetic interactions contribute less than additive effects to quantitative trait variation in yeast.
Genetic interactions contribute less than additive effects to quantitative trait variation in yeast.
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DOI：
10.1038/ncomms9712
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发表时间：
2015-11-05
影响因子：
16.6
通讯作者：
Kruglyak L
中科院分区：
文献类型：
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作者：
Bloom JS;Kotenko I;Sadhu MJ;Treusch S;Albert FW;Kruglyak L
数量性状的遗传作图研究通常集中在检测对性状变异有加性贡献的位点。遗传相互作用通常被认为是性状变异的一个因素，但相互作用对性状变异的相对贡献是一个有争议的问题。在这里，我们使用一个非常大的交叉两个酵母菌株，以准确地估计表型方差的分数，由于成对的QTL-QTL相互作用的20个数量性状。我们发现这个分数平均为9%，大大低于加性QTL的贡献（43%）。统计学显著的QTL-QTL对通常具有较小的个体效应大小，但共同解释了40%的成对互作方差。我们发现，成对的相互作用方差在很大程度上是由成对的位点，其中至少有一个具有显着的加性效应解释。这些结果完善了我们对数量性状遗传结构的理解，并有助于指导未来的作图研究。 这项研究使用了大量的杂交实验室菌株和葡萄园分离的酵母菌株，并估计各种数量性状的表型方差。利用该数据集，作者表明加性数量性状基因座（QTL）和QTL-QTL互作的平均值分别为43%和9%。
Genetic mapping studies of quantitative traits typically focus on detecting loci that contribute additively to trait variation. Genetic interactions are often proposed as a contributing factor to trait variation, but the relative contribution of interactions to trait variation is a subject of debate. Here we use a very large cross between two yeast strains to accurately estimate the fraction of phenotypic variance due to pairwise QTL–QTL interactions for 20 quantitative traits. We find that this fraction is 9% on average, substantially less than the contribution of additive QTL (43%). Statistically significant QTL–QTL pairs typically have small individual effect sizes, but collectively explain 40% of the pairwise interaction variance. We show that pairwise interaction variance is largely explained by pairs of loci at least one of which has a significant additive effect. These results refine our understanding of the genetic architecture of quantitative traits and help guide future mapping studies. This study uses a large number of crosses between a common lab strain and vineyard-isolated strain of yeast, and estimates the phenotypic variance for various quantitative traits. Using this data set, the authors show additive quantitative trait loci (QTL) and QTL–QTL interactions to be on average 43% and 9%, respectively.