Effectiveness of human amnion preserved long-term in glycerol as a temporary biological dressing
Effectiveness of human amnion preserved long-term in glycerol as a temporary biological dressing
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DOI：
10.1016/s0305-4179(99)00072-8
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发表时间：
1999-11-01
期刊：
影响因子：
2.7
通讯作者：
Haberal, M
中科院分区：
文献类型：
--
作者：
Maral, T;Borman, H;Haberal, M
人羊膜作为临时生物伤口敷料仍然是发展中国家治疗烧伤的有益且具有成本效益的手段。本研究的目的是确定与新鲜羊膜和甘油保存的人皮肤相比，在甘油中长期保存的人羊膜是否是一种有效的生物敷料。人羊膜和皮肤样本在 85% 甘油的无菌容器中于 4℃ 保存一年以上。在大鼠身上制造背部全层或裂层皮肤伤口。将缺损分为四个区域，每个区域覆盖有保留羊膜、新鲜羊膜、保留皮肤或不覆盖作为对照。2、4、7、10和14天后对伤口上的材料进行宏观和微观评估。在第 4 天和第 7 天评估移植物在全层伤口上的初次附着或粘附，并根据几个宏观和微观标准对材料性能进行评分。通过定量细菌学检查了大鼠严重感染的全层烫伤创面的细菌水平降低效果。定性培养证实材料经过一年多的储存条件是无菌的，羊膜和皮肤保持了其特性。所有材料均被发现对部分厚度的大鼠伤口有效，作为覆盖物，在其下7天即可完成再上皮化。在全层伤口上，保存的羊膜比保存的羊膜或新鲜的羊膜表现更好，但保存的羊膜的性能与新鲜羊膜的性能相当。研究发现，在降低受感染的大鼠烧伤伤口的细菌水平方面，甘油保存的羊膜与新鲜羊膜或皮肤一样有效。保存在甘油中的羊膜是可靠且长期有效的。羊膜库可以以低成本提供无限量的生物敷料用于烧伤治疗，这一因素在发展中国家尤为重要。 (C) 1999 Elsevier Science Ltd 和 ISBI。版权所有。
Human amnion as a temporary biological wound dressing has remained a beneficial and cost-effective means of treating burns in developing countries. The aim of this study was to determine whether human amnion that has undergone long-term preservation in glycerol is an effective biological dressing compared to fresh amnion and glycerol-preserved human skin.Samples of human amnion and skin were preserved in sterile containers of 85% glycerol at 4 degrees C for over a year. Dorsal full-thickness or split-thickness skin wounds were produced in rats. The defects were divided into four areas, each of which was covered with preserved amnion, fresh amnion, preserved skin, or left uncovered as a control, The materials on the wounds were evaluated macroscopically and microscopically after 2, 4, 7, 10 and 14 days. The primary take or adherence of the grafts on full-thickness wounds was evaluated at 4 and 7 days, and material performance was scored based on several macroscopic and microscopic criteria. The bacteria levels reducing effect of the materials were examined by quantitative bacteriology in heavily infected full-thickness scald burn wounds of rats.Qualitative cultures confirmed that the storage conditions the materials were subjected to for over a year were aseptic and that the amnion and skin had maintained their characteristic properties. All materials were found effective on partial-thickness rat wounds as a cover under which re-epithelialization was completed by 7 days. The preserved skin performed better than either preserved or fresh amnion on full-thickness wounds but the performance of preserved amnion was comparable to that of fresh amnion. Glycerol-preserved amnion was found to be as effective as fresh amnion or skin in terms of decreasing bacterial levels in infected rat burn wounds.Amnion stored in glycerol is reliable and effective for a long period of time. Amnion banking could provide an unlimited quantity of biologic dressing for burn treatment at low cost, a factor that is particularly important in developing countries. (C) 1999 Elsevier Science Ltd and ISBI. All rights reserved.