Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation.
Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation.
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DOI：
10.1155/2015/631369
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发表时间：
2015
影响因子：
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通讯作者：
Takaso M
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文献类型：
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作者：
Yamada T;Uchida K;Onuma K;Inoue G;Aikawa J;Takano S;Sekiguchi H;Fujimaki H;Miyagi M;Takaso M
同种异体骨软骨移植是治疗大软骨缺损的一种很有前途的选择。然而，由于骨软骨组织（OCTs）在4°C的保存过程中细胞活力逐渐降低，因此需要维持新鲜OCTs细胞活力的方法来改善移植结果。在这里，我们评估了在保存液中添加三种不同分子量形式的透明质酸（HA）中的一种是否能提高大鼠OCTs在长期冷藏期间的活力。与未添加UW溶液相比，添加800 kDa的University of Wisconsin （UW）溶液在4°C下加热14天后显著提高OCT细胞活力。相比之下，添加1900或6000 kDa HA的UW溶液并没有显著提高OCT的细胞活力，Real-time PCR分析显示，在添加800 kDa HA的UW溶液中储存的OCT中，基质金属蛋白酶2、3和9的水平显著降低。尽管对人体OCT的进一步研究是必要的，但这些发现表明，使用800 kDa HA代替血清可能是长期保存骨软骨同种异体移植物的合适方法，用于修复临床环境中的大软骨缺损。
Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because the cell viability of osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods for maintaining the cell viability of fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether the supplementation of preservation solution with one of three different molecular weight forms of hyaluronic acid (HA) improved the viability of rat OCTs during long-term cold storage. The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution. In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT. Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA. Although further studies in human OCT are warranted, these findings demonstrate that the use of 800 kDa HA in place of serum may be a suitable approach for the long-term preservation of osteochondral allografts designated for the repair of large cartilage defects in the clinical setting.