TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus.
TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus.
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DOI：
10.1038/srep29430
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发表时间：
2016-07-08
影响因子：
4.6
通讯作者：
Takeda M
中科院分区：
文献类型：
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作者：
Sakai K;Ami Y;Nakajima N;Nakajima K;Kitazawa M;Anraku M;Takayama I;Sangsriratanakul N;Komura M;Sato Y;Asanuma H;Takashita E;Komase K;Takehara K;Tashiro M;Hasegawa H;Odagiri T;Takeda M
甲型流感病毒和B流感病毒在其宿主特异性和大流行潜力方面显示出明显的差异。最近的研究表明，宿主蛋白酶TMPRSS 2在体内对甲型流感病毒（IAV）H1、H3和H7亚型毒株的蛋白水解活化起着至关重要的作用。在血凝素（HA）蛋白中具有单碱基切割位点的IAV在TMPRSS2敲除小鼠中复制较差，这是由于HA切割不足。在本研究中，分析了人分离的B型流感病毒（IBV）毒株和小鼠适应的IBV毒株。数据显示，IBV在TMPRSS 2敲除小鼠中成功地经历了HA裂解，并且小鼠适应株对这些小鼠具有完全致病性。目前的数据表明，IAV和IBV在其体内传播的分子机制有明显的差异。
Influenza A and B viruses show clear differences in their host specificity and pandemic potential. Recent studies have revealed that the host protease TMPRSS2 plays an essential role for proteolytic activation of H1, H3, and H7 subtype strains of influenza A virus (IAV) in vivo. IAV possessing a monobasic cleavage site in the haemagglutinin (HA) protein replicates poorly in TMPRSS2 knockout mice owing to insufficient HA cleavage. In the present study, human isolates of influenza B virus (IBV) strains and a mouse-adapted IBV strain were analysed. The data showed that IBV successfully underwent HA cleavage in TMPRSS2 knockout mice, and that the mouse-adapted strain was fully pathogenic to these mice. The present data demonstrate a clear difference between IAV and IBV in their molecular mechanisms for spreading in vivo.