Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray
Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray
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DOI：
10.1016/j.phytochem.2006.04.010
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发表时间：
2006-08-01
期刊：
影响因子：
3.8
通讯作者：
Cornish, Katrina
中科院分区：
文献类型：
--
作者：
da Costa, Bernardo M. T.;Keasling, Jay D.;Cornish, Katrina
天然橡胶是由橡胶转移酶（顺式异戊二烯转移酶）产生的。橡胶转移酶使用烯丙基焦磷酸来引发橡胶分子，并使用异戊烯基焦磷酸（IPP）来形成聚合物。橡胶生物合成也需要二价金属阳离子。了解分子量是如何调节的很重要，因为高质量的橡胶需要高分子量。我们的特点是在体外的影响，镁+橡胶的生物合成率产生的替代天然橡胶作物，银胶菊（银胶菊）。银胶假单胞菌橡胶转移酶对IPP（.）Mg对Mg ~（2+）浓度的依赖性与H相似。brasiliensis橡胶转移酶，尽管程度不那么极端。此外，在体外Mg 2+浓度显着影响橡胶分子量的两个物种，但分子量是不敏感的P. argentatum比H.巴西的。(c)2006爱思唯尔有限公司保留所有权利。
Natural rubber is produced by a rubber transferase (a cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubber. We characterized the in vitro effects of Mg2+ on the biosynthetic rate of rubber produced by an alternative natural rubber crop, Parthenium argentatum (guayule). The affinity of the rubber transferase from P. argentatum for IPP (.) Mg was shown to depend on the Mg2+ concentration in a similar fashion to the H. brasiliensis rubber transferase, although to a less extreme degree. Also, in vitro Mg2+ concentration significantly affects rubber molecular weight of both species, but molecular weight is less sensitive to Mg2+ concentration in P. argentatum than in H. brasiliensis. (c) 2006 Elsevier Ltd. All rights reserved.