Increased survivin expression in high-grade oral squamous cell carcinoma: a study in Indian tobacco chewers
Increased survivin expression in high-grade oral squamous cell carcinoma: a study in Indian tobacco chewers
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DOI：
10.1111/j.1600-0714.2006.00473.x
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发表时间：
2006-11-01
影响因子：
3.3
通讯作者：
Karjodkar, F. R.
中科院分区：
文献类型：
--
作者：
Jane, C.;Nerurkar, A. V.;Karjodkar, F. R.
背景：口腔癌是印度人口中五大癌症发源地之一。在本研究中，我们分析了细胞凋亡调控基因的表达。Survivin、Bcl2、Bax和P53在印度烟草咀嚼者口腔黏膜癌前病变和癌组织中的表达。方法：采用38例口腔鳞癌和17例白斑石蜡包埋组织标本。结果：36%的口腔鳞癌组织中P53蛋白表达阳性，癌前病变组织中无一例表达P53蛋白。Survivin、Bcl2和Bax的表达随肿瘤恶性程度的升高而升高。Survivin的表达增加在统计学上最为显著(P<；0.001)。结论：抗凋亡的Survivin在高级别肿瘤中的表达增加，提示Survivin可能在治疗后的细胞凋亡抵抗中起重要作用。
Background: Oral cancer is one of the five leading sites of cancer in the Indian population. In the present study we analyzed the expression of apoptosis regulating genes, viz. survivin, Bcl-2, Bax and p53 in precancerous and cancerous lesions of the buccal mucosa of Indian tobacco chewers.Method: Paraffin-embedded tissue samples from 38 patients with primary oral squamous cell carcinoma (OSCC) and 17 patients with leukoplakia were used. The expression of survivin, Bcl-2, Bax, and p53 was evaluated using immunohistochemical staining method.Result: Thirty-six percent OSCC were found to be positive for nuclear p53 staining while none of the precancerous lesions showed p53 positivity. Survivin, Bcl-2 and Bax expression was found to increase with increased grade of malignancy. Increase in survivin expression was statistically most significant (P < 0.001).Conclusion: Increased expression of anti-apoptotic survivin in high-grade tumors suggests that survivin is likely to contribute significantly to apoptosis resistance in response to therapy.