NOVEL PEPTIDOGLYCANS IN CAULOBACTER AND ASTICCACAULIS SPP
NOVEL PEPTIDOGLYCANS IN CAULOBACTER AND ASTICCACAULIS SPP
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DOI：
10.1128/jb.150.1.332-347.1982
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
HAGENZIEKER, JG
中科院分区：
文献类型：
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作者：
POINDEXTER, JS;HAGENZIEKER, JG
不含聚-β-葡聚糖的肽聚糖囊从4种柄杆菌属（Caulobacter spp.）和2个黑茎矮杆藻属（Asticcacaulis spp.）和C. crescentus和C.莱迪。定量分析球囊的酸水解产物，并且每种水解产物仅含有显著量的5种茚三酮反应性化合物：丙氨酸、谷氨酸、α，ε-二氨基庚二酸、胞壁酸和葡糖胺。四种类型的肽聚糖是可区分的基础上，这5种化合物的摩尔比。其比例如下：C. leidyi，2：1：1：1：0.8;在A.双端鞘细胞的比例为1.7：1.6：1：1.1：0.7，其余种细胞的比例为2：1：1：1.2：0.8，而脱落突变体2NY 66脱落的茎的比例为2：1：1：1：1.67。除了这些柄杆菌之间的一些种间差异外，还发现有柄杆菌的肽聚糖小囊与柄杆菌的肽聚糖小囊相似。Crescentus细胞是化学分化的;细胞肽聚糖比典型的革兰氏阴性细菌的肽聚糖更富含胞壁酸，并且柄的肽聚糖相应地富含葡糖胺。肽聚糖的重复单元的经验公式推断的基础上，其氨基组分的摩尔比。
Peptidoglycan sacculi free of poly-.beta.-hydroxybutyric acid were prepared from whole cells of 4 Caulobacter spp. and 2 Asticcacaulis spp. and from morphological mutants of C. crescentus and C. leidyi. Acid hydrolysates of the sacculi were analyzed quantitatively and each of the hydrolysates contains significant amounts of only 5 ninhydrin-reactive compounds: alanine, glutamic acid, .alpha., .epsilon.-diaminopimelic acid, muramic acid and glucosamine. Four types of peptidoglycans were distinguishable on the basis of the molar ratios among these 5 compounds. The respective ratios were as follows: in C. leidyi, 2:1:1:1:0.8; in A. biprosthecum, 1.7:1.6:1:1.1:0.7, in the cells of the remaining species, 2:1:1:1.2:0.8; and in stalks shed by the abscission mutant 2NY66, 2:1:1:1:1.67. In addition to some species differences among these caulobacters, it was found that the peptidoglycan sacculus of the stalked C. crescentus cell is chemically differentiated; the cellular peptidoglycan is richer in muramic acid than is the peptidoglycan of typical gram-negative bacteria and the peptidoglycan of the stalk is correspondingly rich in glucosamine. Empirical formulas for the repeating units of the peptidoglycans were inferred on the basis of the molar ratios of their amino components.