EFFECT OF PROTEIN-SYNTHESIS INHIBITORS ON TESTOSTERONE PRODUCTION IN RAT TESTIS INTERSTITIAL-TISSUE AND LEYDIG-CELL PREPARATIONS
EFFECT OF PROTEIN-SYNTHESIS INHIBITORS ON TESTOSTERONE PRODUCTION IN RAT TESTIS INTERSTITIAL-TISSUE AND LEYDIG-CELL PREPARATIONS
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DOI：
10.1042/bj1500413
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发表时间：
1975-01-01
影响因子：
4.1
通讯作者：
VANDERMOLEN, HJ
中科院分区：
文献类型：
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作者：
COOKE, BA;JANSZEN, FHA;VANDERMOLEN, HJ
黄体生成素刺激的睾酮生物合成抑制放线菌酮在大鼠睾丸间质组织在体外孵育过程中，也由嘌呤霉素和放线菌酮在孵育Leydig细胞制剂，但不是氯霉素。这些结果表明，由细胞质蛋白质合成形成的蛋白质调节剂参与大鼠睾丸类固醇的合成。放线菌酮和嘌呤霉素对蛋白质合成的特异性作用，而不是对其他非特异性过程的建议，抑制蛋白质合成和类固醇合成与不同剂量的抑制剂和缺乏放线菌酮对促黄体激素诱导的腺苷3′：5′-环磷酸生产的影响。促黄体生成素在间质组织灌流期间刺激睾酮产生在10-20分钟内可检测到，并在120分钟达到最大值，此后缓慢下降。以最大类固醇产量加入的环己酰亚胺导致睾酮合成快速减少，其遵循一级动力学（半衰期13分钟），因此表明蛋白质调节剂具有短的半衰期。放线菌酮，嘌呤霉素或氯霉素对睾酮的生产中添加促黄体生成素的情况下，没有发现任何影响，这表明睾酮的基础生产是独立的蛋白质合成。
Luteinizing-hormone-stimulated testosterone biosynthesis was inhibited by cycloheximide during incubation of rat testis intersitial tissue in vitro and also by puromycin and cycloheximide during incubation of Leydig-cell preparations, but not by chloramphenicol. These results suggest that a protein regualtor(s) formed by cytoplasmic protein synthesis is involved in steroidogenesis in the rat testis. The specific effect of cycloheximide and puromycin on protein synthesis rather than on other non-specific processes is suggested by the inhibition of protein synthesis and steroidogenesis with different doses of the inhibitors and the lack of effect of cycloheximide on luteinizing-hormone-induced adenosine 3′:5′-cyclic monophosphate production. Stimulation of testosterone production by luteinizing hormone during superfusion of interstitial tissue was detectable within 10-20 min and reached a maximum of 120 min, and thereafter slowly decreased. Cycloheximide added at maximum steroid production caused a rapid decrease in testosterone synthesis which followed first-order kinetics (half-life 13 min), thus indicating that the protein regulator(s) has a short half-life. No effect of cycloheximide, puromycin or chloramphenicol on testosterone production in the absence of added luteinizing hormone was found, suggesting that the basal production of testosterone is independent of protein synthesis.