A NEW MONOCLONAL-ANTIBODY RECOGNIZING AN ANTIGEN OF HUMAN-LYMPHOCYTES SIMILAR OR IDENTICAL TO TAC ANTIGEN
A NEW MONOCLONAL-ANTIBODY RECOGNIZING AN ANTIGEN OF HUMAN-LYMPHOCYTES SIMILAR OR IDENTICAL TO TAC ANTIGEN
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DOI：
10.1111/j.1348-0421.1984.tb00760.x
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发表时间：
1984-01-01
影响因子：
2.6
通讯作者：
HINUMA, Y
中科院分区：
文献类型：
--
作者：
TANAKA, Y;TOZAWA, H;HINUMA, Y
本文报道了一种新的能与人淋巴细胞表面糖蛋白结合的小鼠单抗(HEI，IgG1型)。膜免疫荧光分析表明，HieI与抗Tac单抗一样，优先与激活的正常人T细胞和携带成人T细胞白血病病毒(ATLV)的人T细胞和B细胞发生反应。然而，HIEI和抗Tac抗体之间的一个有趣的区别是，HIEI不能与ATLV转化的猿猴细胞系或用白介素2(IL-2)培养的细胞发生反应，而抗Tac抗体能。免疫沉淀实验表明，HieI和抗Tac抗体均能从激活的正常T细胞和ATLV阳性的T、B细胞中，通过MT-2共培养的方法，从体外转化的MT-2和MT-2相关的T细胞中，表达一种相对分子质量为60,000道尔顿(Gp60)的糖蛋白。HIEI抑制正常T细胞依赖IL-2的增殖，但其抑制作用远弱于抗Tac抗体。抗Tac抗体干扰HieI与靶细胞的结合，但不阻断抗Tac抗体与细胞的结合。这些观察表明，HIEI抗体识别人类Tac抗原的一个新的抗原决定簇。
A new mouse monoclonal antibody (HEI, IgG1 type) that reacts with a cell surface glycoprotein of human lymphocytes was isolated. Membrane immunofluorescence assay showed that HIEI, like the anti-Tac monoclonal antibody, reacted preferentially with activated normal human T-cells and adult T-cell leukemia virus (ATLV)-carrying human T- and B-cell lines. However, an interesting difference between HIEI and anti-Tac antibody was that HIEI did not react with ATLV-transformed simian cell lines or those cultured with interleukin-2 (IL-2), whereas the anti-Tac antibody did. The immunoprecipitation assay showed that both HIEI and anti-Tac antibody precipitated a glycoprotein with a MW of 60,000 daltons (gp60) from activated normal T-cells and ATLV-positive T- and B-cells and also gp53 from MT-2 and MT-2-related T-cell lines transformed with ATLV in vitro by the MT-2 cocultivation method. HIEI inhibited the IL-2-dependent proliferation of normal T-cells, but its inhibitory effect was much weaker than that of the anti-Tac antibody. The anti-Tac antibody interfered with the binding of HIEI to target cells, but HIEI did not block binding of the anti-Tac antibody to the cells. These observations indicate that HIEI antibody recognizes a new antigenic determinant of the human Tac antigen.