Differential Expression of SNAP-25 Family Proteins in the Mouse Brain
Differential Expression of SNAP-25 Family Proteins in the Mouse Brain
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DOI：
10.1002/cne.22558
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发表时间：
2011-04-01
影响因子：
2.5
通讯作者：
Takahashi, Masami
中科院分区：
文献类型：
--
作者：
Yamamori, Saori;Itakura, Makoto;Takahashi, Masami
可溶性 N-乙基马来酰亚胺敏感因子附着蛋白 (SNAP)-25 是一种神经元 SNARE 蛋白，对于突触前末梢释放神经递质至关重要。三种棕榈酰化 SNAP-25 家族蛋白：SNAP-25a、SNAP-25b 和 SNAP-23 在大脑中表达，但对其分布和功能知之甚少。在本研究中，我们生成了特异性抗体来区分这三种同源蛋白。免疫印迹和免疫组织化学分析表明，SNAP-25b分布在几乎整个大脑的突触富集区域，而SNAP-25a和SNAP-23表达在相对特定的大脑区域，表达模式部分互补。 SNAP-25a 和 SNAP-25b，但不包括 SNAP-23，也存在于神经纤维的轴浆中。细胞内定位也不同，虽然 SNAP-25b 和 SNAP-23 主要存在于小鼠脑匀浆的膜和脂筏富集部分中，但在可溶性部分中回收了大量的 SNAP-25a。在 PC12 细胞中，SNAP-25b 定位于质膜，但 SNAP-25a 和 SNAP-23 分布在整个细胞质中。这三种蛋白的表达和分布在出生后早期也受到差异性调节。这些结果表明，三个 SNAP-25 家族蛋白在大脑和神经元细胞中表现出不同的分布，并且可能发挥不同的作用。 J.Comp。内罗尔。 519:916-932, 2011。(C) 2010 Wiley-Liss, Inc.
Soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP)-25 is a neuronal SNARE protein essential for neurotransmitter release from presynaptic terminals. Three palmitoylated SNAP-25 family proteins: SNAP-25a, SNAP-25b, and SNAP-23, are expressed in the brain, but little is known about their distributions and functions. In the present study, we generated specific antibodies to distinguish these three homologous proteins. Immunoblot and immunohistochemical analyses revealed that SNAP-25b was distributed in synapse-enriched regions throughout almost the entire brain, whereas SNAP-25a and SNAP-23 were expressed in relatively specific brain regions with partially complementary expression patterns. SNAP-25a and SNAP-25b, but not SNAP-23, were also present in the axoplasm of nerve fibers. The intracellular localization was also different, and although SNAP-25b and SNAP-23 were found primarily in membrane and lipid raft-enriched fractions of mouse brain homogenates, a substantial amount of SNAP-25a was recovered in soluble fractions. In PC12 cells, SNAP-25b was localized to the plasma membrane, but SNAP-25a and SNAP-23 were distributed throughout the cytoplasm. The expression and distribution of these three proteins were also differentially regulated in the early postnatal period. These results indicate that the three SNAP-25 family proteins display a differential distribution in the brain as well as in neuronal cells, and possibly play distinct roles. J. Comp. Neurol. 519:916-932, 2011. (C) 2010 Wiley-Liss, Inc.