Inhibition of tooth eruption in the rat by a bisphosphonate
Inhibition of tooth eruption in the rat by a bisphosphonate
复制标题
DOI：
10.1177/00220345980770011201
复制
发表时间：
1998-01-01
影响因子：
7.6
通讯作者：
Wise, GE
中科院分区：
文献类型：
--
作者：
Grier, RL;Wise, GE
对骨硬化啮齿动物的研究表明，如果牙齿要萌出，必须发生局部牙槽骨吸收。为了直接验证这一假设，我们给出生后的大鼠注射了帕米膦酸盐，这是一种双磷酸盐，可以减少破骨细胞的骨吸收。这些实验的结果表明，这种双膦酸盐抑制大鼠磨牙和门牙的牙齿萌出时间。帕米膦酸盐不抑制假定的牙齿萌出分子，集落刺激因子-1和c-fas的基因表达，这两种分子都在牙囊中表达，牙囊是萌出所需的组织。帕米膦酸盐确实增加了破骨细胞的大小，包括细胞核数量的增加，这表明尽管破骨细胞再吸收骨的能力降低，但前体单核细胞仍然可以融合形成破骨细胞。因此，我们报告发现一种抑制牙齿萌出的药物，并表明牙齿萌出需要牙槽骨吸收。
Studies of osteopetrotic rodents suggest that localized alveolar bone resorption must occur if the tooth is to erupt. To test this hypothesis directly, we injected postnatal rats with pamidronate, a bisphosphonate that reduces bone resorption by osteoclasts. The results of these experiments demonstrate that this bisphosphonate inhibits the time of tooth eruption of both rat molars and incisors. Pamidronate does not inhibit the gene expression of the putative tooth eruption molecules, colony-stimulating factor-1 and c-fas, both of which are expressed in the dental follicle, the tissue that is required for eruption to occur. Pamidronate does increase the size of the osteoclasts, including an increase in the number of nuclei, suggesting that the precursor mononuclear cells can still fuse to form osteoclasts despite the reduced ability of the osteoclasts to resorb bone. Thus, we report the discovery of an agent that inhibits tooth eruption and also show that tooth eruption requires alveolar bone resorption.