Overexpression of EaDREB2 and pyramiding of EaDREB2 with the pea DNA helicase gene (PDH45) enhance drought and salinity tolerance in sugarcane (Saccharum spp. hybrid)
Overexpression of EaDREB2 and pyramiding of EaDREB2 with the pea DNA helicase gene (PDH45) enhance drought and salinity tolerance in sugarcane (Saccharum spp. hybrid)
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DOI：
10.1007/s00299-014-1704-6
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发表时间：
2015-02-01
影响因子：
6.2
通讯作者：
Subramonian, N.
中科院分区：
文献类型：
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作者：
Augustine, Sruthy Maria;Narayan, J. Ashwin;Subramonian, N.
EaDREB 2在甘蔗中的过表达增强了对干旱和盐的耐受性。当与植物DNA解旋酶基因共转化时，DREB 2表现出比单基因转基因更高的耐盐性。干旱是限制甘蔗可持续生产的最具挑战性的农业问题之一，可能导致高达50%的产量损失。DREB蛋白在植物的非生物胁迫耐受性中起着重要的调节作用。我们先前报道了干旱胁迫增强斑茅中EaDREB 2的表达。本研究从大肠杆菌中克隆了DREB 2基因。将EaDREB 2基因转化到印度热带甘蔗品种Co 86032上，通过基因枪法将EaDREB 2基因与Port Ubi 2.3启动子驱动的PDH 45基因进行了融合，并对获得的V-1转基因植株进行了水分胁迫和盐胁迫的抗性鉴定。在分蘖期采用隔水灌溉方式施加土壤水分胁迫。利用生理、分子和形态学参数评价其耐旱性。通过盐胁迫下的叶盘衰老和芽萌发试验来评价耐盐性。我们的研究结果表明，EaDREB 2在甘蔗中的过表达增强耐旱性和耐盐性在更大程度上比未转化的对照植物。这是首次报道EaDREB 2和PDH 45共转化的结果，与EaDREB 2单独转化相比，其表现出更高的耐盐性，但更低的耐旱性。本研究似乎表明，为了将耐旱性和耐盐性结合在一起，共转化是一种更好的方法。
EaDREB2 overexpressed in sugarcane enhanced tolerance to drought and salinity. When co-transformed with plant DNA helicase gene, DREB2 showed greater level of salinity tolerance than in single-gene transgenics.Drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and can potentially cause up to 50 % yield loss. DREB proteins play a vital regulatory role in abiotic stress tolerance in plants. We previously reported that expression of EaDREB2 is enhanced by drought stress in Erianthus arundinaceus. In this study, we have isolated the DREB2 gene from E. arundinaceus, transformed one of the most popular sugarcane variety Co 86032 in tropical India with EaDREB2 through Agrobacterium-mediated transformation, pyramided the EaDREB2 gene with the gene coding for PDH45 driven by Port Ubi 2.3 promoter through particle bombardment and evaluated the V-1 transgenics for soil deficit moisture and salinity stresses. Soil moisture stress was imposed at the tillering phase by withholding irrigation. Physiological, molecular and morphological parameters were used to assess drought tolerance. Salinity tolerance was assessed through leaf disc senescence and bud sprout assays under salinity stress. Our results indicate that overexpression of EaDREB2 in sugarcane enhances drought and salinity tolerance to a greater extent than the untransformed control plants. This is the first report of the co-transformation of EaDREB2 and PDH45 which shows higher salinity tolerance but lower drought tolerance than EaDREB2 alone. The present study seems to suggest that, for combining drought and salinity tolerance together, co-transformation is a better approach.