STRONG EVOLUTIONARY CONSERVATION OF NEUROPEPTIDE-Y - SEQUENCES OF CHICKEN, GOLDFISH, AND TORPEDO-MARMORATA DNA CLONES
STRONG EVOLUTIONARY CONSERVATION OF NEUROPEPTIDE-Y - SEQUENCES OF CHICKEN, GOLDFISH, AND TORPEDO-MARMORATA DNA CLONES
复制标题
DOI：
10.1073/pnas.89.6.2350
复制
发表时间：
1992-03-15
影响因子：
11.1
通讯作者：
LARHAMMAR, D
中科院分区：
文献类型：
--
作者：
BLOMQVIST, AG;SODERBERG, C;LARHAMMAR, D
神经肽 Y (NPY) 是哺乳动物神经系统中丰富且广泛分布的神经肽。 NPY 属于 36 个氨基酸肽家族，该家族还包括胰多肽、内分泌肠肽 YY 以及鱼胰肽 Y。为了研究该肽家族的进化，我们从鸡、金鱼和鳐鱼雷鱼雷鱼的中枢神经系统 cDNA 文库以及鸡基因组文库中分离了编码 NPY 的克隆。预测的鸡 NPY 氨基酸序列与大鼠的仅在一个位置上不同。金鱼序列在五个位置上不同，表明硬骨鱼除了胰肽 Y 之外还具有真正的 NPY 肽。Torpedo 序列与大鼠序列在三个位置上不同。由于Torpedo NPY与其他序列相比没有独特的位置，因此它似乎与经过4.2亿年进化的软骨鱼、硬骨鱼和四足动物共同祖先的NPY相同。 NPY 前体的 30 个氨基酸羧基末端延伸也显示出相当大的序列保守性。这些结果表明 NPY 是最高度保守的神经内分泌肽之一。
Neuropeptide Y (NPY) is an abundant and widespread neuropeptide in the nervous system of mammals. NPY belongs to a family of 36-amino acid peptides that also includes pancreatic polypeptide and the endocrine gut peptide YY as well as the fish pancreatic peptide Y. To study the evolution of this peptide family, we have isolated clones encoding NPY from central nervous system cDNA libraries of chicken, goldfish, and the ray Torpedo marmorata, as well as from a chicken genomic library. The predicted chicken NPY amino acid sequence differs from that of rat at only one position. The goldfish sequence differs at five positions and shows that bony fishes have a true NPY peptide in addition to their pancreatic Peptide Y. The Torpedo sequence differs from that of rat at three positions. As Torpedo NPY has no unique positions when compared with the other sequences, it seems to be identical to the NPY of the common ancestor of cartilaginous fishes, bony fishes, and tetrapods after 420 million years of evolution. The 30-amino acid carboxyl-terminal extension of the NPY precursor also displays considerable sequence conservation. These results show that NPY is one of the most highly conserved neuroendocrine peptides.