Optimization of β-carotene production by Rhodotorula glutinis DM28 in fermented radish brine
Optimization of β-carotene production by Rhodotorula glutinis DM28 in fermented radish brine
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DOI：
10.1016/j.biortech.2007.05.019
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发表时间：
2008-05-01
影响因子：
11.4
通讯作者：
Suntornsuk, W.
中科院分区：
文献类型：
--
作者：
Malisorn, C.;Suntornsuk, W.
采用面心中心复合设计来优化培养条件，以提高粘红酵母 DM28 在搅拌釜反应器中的 P-胡萝卜素产量，并使用 30 g/l 总可溶性固体的发酵萝卜盐水作为唯一底物。实验采用回归模型进行，其中温度、pH 值和溶解氧被视为变量。结果表明，酵母生产β-胡萝卜素的最佳条件为30℃、pH 6、溶解氧80%。在此条件下，发酵24小时后，酵母产生2.7克/升的生物量和最大201微克/升的β-胡萝卜素，表明比初始条件下的生物量（分别为2.3克/升和178微克/升）高约15%。 (c) 2007 Elsevier Ltd. 保留所有权利。
A face-centered central composite design was applied to optimize a cultivation condition for improved P-carotene production by Rhodotorula glutinis DM28 in a stirred tank reactor using 30 g/l total soluble solid of fermented radish brine as a sole substrate. The experiments were performed with regression models, where temperature, pH and dissolved oxygen were considered as variables. Results showed that an optimum condition for P-carotene production of the yeast was at 30 degrees C, pH 6 and 80% dissolved oxygen. Under this condition, the yeast yielded 2.7 g/l biomass and the maximum P-carotene of 201 mu g/l after 24-h fermentation indicating approximately 15% higher than those under an initial condition (2.3 g/l and 178 mu g/l, respectively). (c) 2007 Elsevier Ltd. All rights reserved.