Family with sequence similarity 83 member A promotes tumor cell proliferation and metastasis and predicts poor prognosis in cervical cancer
Family with sequence similarity 83 member A promotes tumor cell proliferation and metastasis and predicts poor prognosis in cervical cancer
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具有序列相似性的家族83成员A促进肿瘤细胞增殖和转移，并预测宫颈癌的不良预后。
DOI：
10.1016/j.prp.2021.153450
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发表时间：
2021-05-04
影响因子：
2.8
通讯作者：
Yao, Shuzhong
中科院分区：
文献类型：
--
作者：
Liu, Tianyu;Chen, Jian;Yao, Shuzhong
具有序列相似性的家族83成员A（FAM 83 A）是FAM 83家族的成员，并且被证明在几种癌症中具有致癌特性。然而，FAM 83 A在人类宫颈癌（CC）进展中的机制尚不清楚。在此，我们通过western blot和qRT-PCR发现FAM 83 A在CC组织和细胞系中高度表达。我们利用GEO数据集来评估与正常宫颈组织（NCT）（GSE 6791）相比CC中的FAM 83 A表达，并且类似地，与淋巴结阴性CC（GSE 26511）相比淋巴结阳性CC中的FAM 83 A表达。使用免疫组织化学（IHC）来定量20个NCT和105个CC患者样品中的FAM 83 A表达。FAM 83 A表达在早期CC中上调，并与侵袭性临床病理特征相关。此外，我们医院和TCGA数据集均显示，FAM 83 A高表达的早期CC患者预后较差。随后，CCK-8和transwell测定证实FAM 83 A促进CC细胞的增殖、迁移和侵袭。此外，基因集富集分析（GSEA）揭示FAM 83 A不仅参与细胞发育、分化和增殖，而且还与细胞连接组装和细胞基质粘附相关。可能与肿瘤坏死因子介导的信号通路和ErbB信号通路的调节有关。这些结果表明FAM 83 A促进肿瘤细胞增殖、迁移和转移。我们的研究提供了新的证据FAM 83 A可能作为一个有前途的治疗CC的目标。
Family with sequence similarity 83 member A (FAM83A) is a member of the FAM83 family and is proven to have oncogenic properties in several cancers. However, the mechanisms of FAM83A in human cervical cancer (CC) progression are unknown. Here, we found that FAM83A is highly expressed in CC tissues and cell lines through western blot and qRT-PCR. We utilized GEO datasets to assess FAM83A expression in CC in comparison to the normal cervical tissue (NCT) (GSE6791), and similarly, in lymph node positive CC compared to the lymph node negative CC (GSE26511). Immunohistochemistry (IHC) was used to quantify FAM83A expression in 20 NCT and 105 CC patient samples. FAM83A expression is upregulated in early-stage CC and correlates with aggressive clinicopathologic features. Moreover, both our hospital's and TCGA datasets revealed that patients of early-stage CC with higher FAM83A expression had a poorer prognosis. Subsequently, CCK-8 and transwell assays verified that FAM83A promotes proliferation, migration, and invasion of CC cells. Additionally, Gene Set Enrichment Analysis (GSEA) revealed that FAM83A is not only involved in cell development, differentiation, and proliferation but is also correlated with cell junction assembly and cell matrix adhesion. It might also be affiliated with the regulation of tumor necrosis factor-mediated signaling pathway and the regulation of the ErbB signaling pathway in CC. These results indicate that FAM83A promotes tumor cell proliferation, migration, and metastasis. Our study provides novel evidence FAM83A may act as a promising therapeutic target for CC.