FIBRONECTIN IN THE DEVELOPMENT OF EMBRYONIC CHICK EYE
FIBRONECTIN IN THE DEVELOPMENT OF EMBRYONIC CHICK EYE
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DOI：
10.1016/0012-1606(79)90313-0
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
SAXEN, L
中科院分区：
文献类型：
--
作者：
KURKINEN, M;ALITALO, K;SAXEN, L
用间接免疫荧光法研究了鸡胚眼内纤维连接蛋白的出现和分布的时间过程。在12体节阶段，纤维连接蛋白是目前作为一个层下的外胚层细胞覆盖前脑泡和头部间充质。在透镜基板形成及其内陷过程中，含有纤维连接蛋白的区域作为囊的组成部分持续存在于透镜周围。在脱细胞基质形成的过程中，含纤维连接蛋白的层与角膜上皮细胞分离。迁移的角膜内皮细胞位于纤维连接蛋白层的后方。次级间质纤维连接蛋白强阳性。纤维连接蛋白从角膜后部开始沿着角膜冷凝物消失。在新生鸡角膜中，纤维连接蛋白仅存在于德斯密氏膜中。胚胎玻璃体有一个含纤维连接蛋白的网状结构。纤连蛋白在发育中的角膜中的分布和关于这种糖蛋白的其他可用数据表明纤连蛋白在细胞的定位和迁移以及细胞外基质的组织中的作用。
The time course of appearance and distribution of fibronectin in the developing eye were studied in chick embryos by indirect immunofluorescence. At the 12-somite stage, fibronectin was present as a layer under the ectodermal cells overlying the forebrain vesicle and in the head mesenchyme. During formation of the lens placode and its invagination, a zone containing fibronectin persisted around the lens as a component of the capsule. The fibronectin-containing layer was separated from the corneal epithelial cells during the formation of the acellular stroma. The migrating corneal endothelial cells were posterior to the fibronectin layer. The secondary stroma was strongly positive for fibronectin. Fibronectin disappeared form the cornea starting from its posterior part along with the corneal condensation. In the newborn chicken cornea, fibronectin was present only in Descemet''s membrane. The embryonic vitreous body had a network of fibronectin-containing material. The distribution of fibronectin in the developing cornea and other data available on this glycoprotein indicate the role of fibronectin in positioning and migration of cells and in organization of the extracellular matrix.