Parallel recognition of cancer cells using an addressable array of solid-state micropores
Parallel recognition of cancer cells using an addressable array of solid-state micropores
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DOI：
10.1016/j.bios.2014.06.048
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发表时间：
2014-12-15
影响因子：
12.6
通讯作者：
Iqbal, Samir M.
中科院分区：
文献类型：
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作者：
Ilyas, Azhar;Asghar, Waseem;Iqbal, Samir M.
癌症患者外周血中循环肿瘤细胞（CTC）的早期检测和精确定量对于早期诊断是重要的。早期诊断可提高治疗效果，改善预后。几种技术已用于CTC检测，但受限于它们需要染料标记、低通量和缺乏单细胞水平的统计可靠性。固态微孔可以表征样品中的每个细胞，提供有关细胞群体的有趣信息。我们报告了一种利用固态微孔阵列组件同时测量细胞移位的多通道装置。这增加了测量的通量，并且当细胞通过微孔时，与白细胞相比，肿瘤细胞显示出独特的电流阻断脉冲。连续监测并记录通过每个跨膜通道的离子电流。该测量系统不仅提高了吞吐量，而且还提供了片上交叉关系。将全血裂解以除去红细胞，因此不需要血液稀释。该方法有助于更快地处理血液样本，肿瘤细胞检测效率约为70%。该设计提供了一种简单且廉价的方法，可以快速可靠地检测肿瘤细胞，无需任何细胞染色或表面功能化。该装置也可用于其他细胞类型的高通量电生理分析。(C)2014爱思唯尔有限公司版权所有。
Early stage detection and precise quantification of circulating tumor cells (CTCs) in the peripheral blood of cancer patients are important for early diagnosis. Early diagnosis improves the effectiveness of the therapy and results in better prognosis. Several techniques have been used for CTC detection but are limited by their need for dye tagging, low throughput and lack of statistical reliability at single cell level. Solid-state micropores can characterize each cell in a sample providing interesting information about cellular populations. We report a multi-channel device which utilized solid-state micropores array assembly for simultaneous measurement of cell translocation. This increased the throughput of measurement and as the cells passed the micropores, tumor cells showed distinctive current blockade pulses, when compared to leukocytes. The ionic current across each micropore channel was continuously monitored and recorded. The measurement system not only increased throughput but also provided on-chip cross-relation. The whole blood was lysed to get rid of red blood cells, so the blood dilution was not needed. The approach facilitated faster processing of blood samples with tumor cell detection efficiency of about 70%. The design provided a simple and inexpensive method for rapid and reliable detection of tumor cells without any cell staining or surface functionalization. The device can also be used for high throughput electrophysiological analysis of other cell types. (C) 2014 Elsevier B.V. All rights reserved.