Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity
Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity
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受体样细胞质激酶的质膜-核-质协调促进EDS1依赖性植物免疫
DOI：
10.1038/s41477-022-01195-x
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发表时间：
2022-07-18
期刊：
影响因子：
18
通讯作者：
Li, Jian-Feng
中科院分区：
文献类型：
--
作者：
Li, Yujia;Xue, Jiao;Li, Jian-Feng
植物利用细胞表面免疫受体识别病原体特异性模式，以唤起基础免疫。增强的疾病易感性（EDS 1）是已知的植物基础免疫的关键，而其激活机制的模式识别仍然是一个谜。在这里，我们表明，真菌模式几丁质诱导质膜锚定受体样胞质激酶PBS 1-LIKE 19（PBL 19）进行核转位在拟南芥。棕榈酰化缺陷型PBL 19（C3 A）变异体始终存在于细胞核中，主要通过WRKY 8和EDS 1依赖的组成性免疫触发转录自我扩增。出乎意料的是，缺乏N-末端核定位序列的后半胱氨酸蛋白酶切割的PBL 19特异性地与细胞质中的EDS 1相互作用并磷酸化。磷酸缺陷型EDS 1减弱了PBL 19（C3 A）诱导的组成性免疫，而磷酸模拟EDS 1补充了PBL 19的真菌抗性损失。PBL 19的质膜、细胞核和细胞质库在时间上协调了免疫信号接收器、放大器和效应器的不同作用，通过EDS 1增强植物抗真菌免疫。病原体激发子如何激活植物免疫信号中心EDS 1仍是一个谜。Li等人表明，真菌诱导子感知可以在优雅的亚细胞协调后通过质膜栓系激酶诱导EDS 1磷酸化。
Plants use cell-surface immune receptors to recognize pathogen-specific patterns to evoke basal immunity. ENHANCED DISEASE SUSCEPTIBILITY (EDS1) is known to be crucial for plant basal immunity, whereas its activation mechanism by pattern recognition remains enigmatic. Here, we show that the fungal pattern chitin induced the plasma membrane-anchored receptor-like cytoplasmic kinase PBS1-LIKE 19 (PBL19) to undergo nuclear translocation in Arabidopsis. The palmitoylation-deficient PBL19(C3A) variant constantly resided in the nucleus, triggering transcriptional self-amplification mainly through WRKY8 and EDS1-dependent constitutive immunity. Unexpectedly, the metacaspase-cleaved PBL19 lacking the N-terminal nuclear localization sequence specifically interacted with and phosphorylated EDS1 in the cytoplasm. Phosphodeficient EDS1 attenuated PBL19(C3A)-induced constitutive immunity, while phosphomimetic EDS1 complemented the loss of PBL19 for fungal resistance. Collectively, these findings reveal a compelling model wherein the plasma membrane, nuclear and cytoplasmic pools of PBL19 temporally coordinate distinct roles of immune signal receiver, amplifier and effector to boost plant antifungal immunity via EDS1.How the plant immune signalling hub EDS1 is activated by pathogen elicitors remains enigmatic. Li et al. show that fungal elicitor perception can induce EDS1 phosphorylation by a plasma membrane tethering kinase after elegant subcellular coordination.