31P MR spectroscopy and in vitro markers of oxidative capacity in type 2 diabetes patients
31P MR spectroscopy and in vitro markers of oxidative capacity in type 2 diabetes patients
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DOI：
10.1007/s10334-006-0060-0
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发表时间：
2006-12-01
影响因子：
2.3
通讯作者：
Prompers, J. J.
中科院分区：
文献类型：
--
作者：
Praet, S. F. E.;De Feyter, H. M. M.;Prompers, J. J.
背景：2型糖尿病（T2 D）患者骨骼肌线粒体功能目前正在深入研究。在体P-31磁共振波谱（P-31 MRS）是一种无创性的测量骨骼肌组织线粒体呼吸功能（MIFU）的工具。然而，在长期的T2 D微血管共病可以干扰P-31 MRS methodology.Aim：比较P-31 MRS衍生参数描述在体内MIFU与体外评估肌肉呼吸能力和肌纤维型composition in T2 D patients.Methods：P-31 MRS被应用于长期的，胰岛素治疗的T2 D患者。在M.股外侧肌肌活检标本收集从同一肌肉和分析琥珀酸脱氢酶活性（SDH）和纤维型distribution.Results：几个P-31 MRS参数的MIFU显示中度至良好的相关性与I型纤维和I型纤维特异性SDH活性的百分比（Pearson的R在0.70和0.75之间）。在体内和体外参数的局部线粒体呼吸也相关性很好，与全身健身水平（VO 2 peak）在这些patients（皮尔逊的R之间的0.62和0.90）。结论：良好的相关性存在于体内和体外测量的MIFU在长期胰岛素治疗的T2 D受试者，这是定性和定量与以前的结果在健康受试者中测量。这证明了使用31 P MRS测量与T2 D相关的MIFU是合理的。
Background: Skeletal muscle mitochondrial function in type 2 diabetes (T2D) is currently being studied intensively. In vivo P-31 magnetic resonance spectroscopy (P-31 MRS) is a noninvasive tool to measure mitochondrial respiratory function (MIFU) in skeletal muscle tissue. However, microvascular co-morbidity in long-standing T2D can interfere with the P-31 MRS methodology.Aim: To compare P-31 MRS-derived parameters describing in vivo MIFU with an in vitro assessment of muscle respiratory capacity and muscle fiber-type composition in T2D patients.Methods: P-31 MRS was applied in long-standing, insulin-treated T2D patients. P-31 MRS markers of MIFU were measured in the M. vastus lateralis. Muscle biopsy samples were collected from the same muscle and analyzed for succinate dehydrogenase activity (SDH) and fiber-type distribution.Results: Several P-31 MRS parameters of MIFU showed moderate to good correlations with the percentage of type I fibers and type I fiber-specific SDH activity (Pearson's R between 0.70 and 0.75). In vivo and in vitro parameters of local mitochondrial respiration also correlated well with whole-body fitness levels (VO2peak) in these patients (Pearson's R between 0.62 and 0.90).Conclusion: Good correlations exist between in vivo and in vitro measurements of MIFU in long-standing insulin-treated T2D subjects, which are qualitatively and quantitatively consistent with previous results measured in healthy subjects. This justifies the use of 31P MRS to measure MIFU in relation to T2D.