Diverse VH and VL genes are used to produce antibodies against a defined protein epitope.
Diverse VH and VL genes are used to produce antibodies against a defined protein epitope.
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不同的 VH 和 VL 基因用于产生针对特定蛋白质表位的抗体。
作者：
M. L. Andria;S. Levy;E. Benjamini
用模型抗原烟草花叶病毒蛋白免疫小鼠，获得分泌单抗的杂交瘤细胞。筛选出6株能与烟草花叶病毒蛋白103-112和97-107氨基酸残基对应的合成肽结合的单抗。通过测定与合成的十肽类似物的结合，分析了这些单抗的良好特异性，并测定了编码单抗V区的cDNA序列。这些分析表明，广泛的不同的V区能够与相同的十肽表位结合，这些抗体序列的差异通常与不同的结合精细特异性相吻合。这种对同一表位具有特异性的不同抗体反应既表明了免疫系统潜力的广度，也表明了特定蛋白质：蛋白质相互作用的缺乏排他性。
mAb secreting hybridomas were produced from mice hyperimmune to the model Ag tobacco mosaic virus protein. Six mAb were selected for their ability to bind synthetic peptides corresponding to amino acid residues 103-112 and 97-107 of tobacco mosaic virus protein. These mAb were analyzed for their fine specificity by measuring binding to synthetic analogs of the decapeptide, and cDNA sequences encoding the mAb V regions were determined. These analyses revealed that a wide range of different V regions are capable of binding with the same decapeptide epitope, and that these antibody sequence differences generally coincided with different binding fine specificities. This diverse antibody response with specificity for the same epitope demonstrates both the breadth of potential of the immune system and the lack of exclusivity in specific protein:protein interactions.