The effect of aging on dopamine release and metabolism during sevoflurane anesthesia in rat striatum: An in vivo microdialysis study
The effect of aging on dopamine release and metabolism during sevoflurane anesthesia in rat striatum: An in vivo microdialysis study
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DOI：
10.1016/j.brainresbull.2012.08.006
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发表时间：
2012-12-01
影响因子：
3.8
通讯作者：
Matsuda, Naoyuki
中科院分区：
文献类型：
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作者：
Kimura-Kuroiwa, Kaori;Adachi, Yushi U.;Matsuda, Naoyuki
我们以前报道过，氟烷麻醉增加了大鼠纹状体中多巴胺（DA）代谢物的细胞外浓度。衰老会导致大脑发生许多变化，包括神经传递。然而，在吸入麻醉中，衰老与神经递质释放变化之间的关系尚未得到充分的研究。本研究旨在评价七氟醚对甲基苯丙胺（MAPT）诱导的中青年大鼠DA释放和代谢的影响。在雄性sd大鼠右侧纹状体内植入微透析探针。用改良的林格氏液灌注探针，每隔20 min直接将透析液40 μ l注入高效液相色谱。大鼠分别给予生理盐水、等量2 mg kg(-1) MAPT腹腔灌胃或5 μ M MAPT局部灌注。治疗后，用1%或3%七氟醚麻醉大鼠1小时。七氟醚麻醉仅在52周龄的中年大鼠中显著增加DA的细胞外浓度。在幼龄大鼠（8周龄）中，七氟醚在腹腔和局部灌注时显著增强了mapt诱导的DA，而在中年大鼠中没有发现显著的加性相互作用。这些结果表明，衰老主要通过降低DA转运体来改变大鼠脑内DA的释放和代谢。(C) 2012爱思唯尔公司版权所有。
We have previously reported that halothane anesthesia increases extracellular concentrations of dopamine (DA) metabolites in rat striatum using in vivo microdialysis techniques. Aging induces many changes in the brain, including neurotransmission. However, the relationship between aging and changes in neurotransmitter release during inhalational anesthesia has not been fully investigated. The aim of the present investigation was to evaluate the effect of sevoflurane on methamphetamine (MAPT)-induced DA release and metabolism in young and middle-aged rats. Male Sprague-Dawley rats were implanted with a microdialysis probe into the right striatum. The probe was perfused with a modified Ringer's solution and 40 mu l of dialysate was directly injected to an HPLC every 20 min. Rats were administered saline, the same volume of 2 mg kg(-1) MAPT intraperitoneally, or 5 mu M MAPT locally perfused. After treatments, the rats were anesthetized with 1% or 3% sevoflurane for 1 h. Sevoflurane anesthesia significantly increased the extracellular concentration of DA only in middle-aged rats (52-weeks-old). In young rats (8-weeks-old), sevoflurane significantly enhanced MAPT-induced DA when administered both intraperitoneally and perfused locally, whereas no significant additive interaction was found in middle-aged rats. These results suggest that aging changes DA release and metabolism in rat brains primarily by decreasing the DA transporter. (C) 2012 Elsevier Inc. All rights reserved.