S100A8/A9 regulates MMP-2 expression and invasion and migration by carcinoma cells.
S100A8/A9 regulates MMP-2 expression and invasion and migration by carcinoma cells.
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DOI：
10.1016/j.biocel.2014.09.007
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发表时间：
2014-10
影响因子：
4
通讯作者：
Herzberg, Mark C.
中科院分区：
文献类型：
--
作者：
Silva, Emmanuel J.;Argyris, Prokopios P.;Zou, Xianqiong;Ross, Karen F.;Herzberg, Mark C.
Intracellular calprotectin (S100A8/A9) functions in the control of the cell cycle checkpoint at G2/M. Dysregulation of S100A8/A9 appears to cause loss of the checkpoint, which frequently characterizes head and neck squamous cell carcinoma (HNSCC). In the present study, we analyzed carcinoma cells for other S100A8/A9-directed changes in malignant phenotype. Using a S100A8/A9-negative human carcinoma cell line (KB), transfection to express S100A8 and S100A9 caused selective down-regulation of MMP-2 and inhibited in vitro invasion and migration. Conversely, silencing of endogenous S100A8 and S100A9 expression in TR146 cells, a well-differentiated HNSCC cell line, increased MMP-2 activity and in vitro invasion and migration. When MMP-2 expression was silenced, cells appeared to assume a less malignant phenotype. To more closely model the architecture of cell growth in vivo, cells were grown in a 3D collagen substrate, which was compared to 2D. Growth on 3D substrates caused greater MMP-2 expression. Whereas hypermethylation of CpG islands occurs frequently in HNSCC, S100A8/A9-dependent regulation of MMP-2 could not be explained by modification of the upstream promoters of MMP2 or TIMP2. Collectively, these results suggest that intracellular S100A8/A9 contributes to the cancer cell phenotype by modulating MMP-2 expression and activity to regulate cell migration and mobility.
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影响因子：
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作者：
Champaiboon, Chantrakorn;Sappington, Kaia J.;Herzberg, Mark C.
通讯作者：
Herzberg, Mark C.
影响因子：
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6.6
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