Effect of lactate and pH on mouse pluripotent stem cells: Importance of media analysis
Effect of lactate and pH on mouse pluripotent stem cells: Importance of media analysis
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DOI：
10.1016/j.bej.2016.11.005
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发表时间：
2017-02-15
影响因子：
3.9
通讯作者：
Verma, Paul
中科院分区：
文献类型：
--
作者：
Gupta, Priyanka;Hourigan, Kerry;Verma, Paul
多能干细胞在临床治疗和再生医学中的应用引起了极大的兴奋。与其他哺乳动物细胞类似，这些细胞的生长取决于各种培养条件。据报道，乳酸作为代谢副产物的存在在大多数情况下对哺乳动物细胞是有害的。然而，对于某些细胞类型，也已经报道了使用乳酸盐作为能量来源。目前的研究进行检查的影响，培养基乳酸浓度和细胞外pH值的变化，发生由于乳酸的生产，对小鼠多能干细胞，胚胎和诱导的多能干细胞的两个重要参数。我们对饲养层依赖性和非饲养层依赖性胚胎干细胞系进行了全面的观察。注意到pH的升高或降低影响所有三种细胞系的细胞增殖、活力和多能性。乳酸盐的作用不太明显。补充乳酸盐降低细胞增殖和细胞数量。然而，从获得的数据，我们假设存活的细胞能够适应环境的变化，并利用乳酸作为能源。乳酸盐对细胞的多能性没有显著影响。(C)2016爱思唯尔B.V.保留所有权利。
Pluripotent stem cells have generated a great deal of excitement concerning their use for clinical therapy and regenerative medicine. Similar to other mammalian cells, the growth of these cells is dependent on various culture conditions. The presence of lactic acid as a metabolic by-product has been reported to be detrimental for mammalian cells in most cases. However, the use of lactate as a source of energy has also been reported for certain cell types. The current study was carried out to examine the effect of media lactate concentration and extracellular pH change, two important parameters that occur due to lactic acid production, on mouse pluripotent stem cells, both embryonic and induced pluripotent stem cells. We examined both feeder dependent and feeder independent embryonic stem cell lines for a comprehensive observation. It was noted that increase or decrease in pH affected cell proliferation, viability and pluripotency of all three cell lines. The effect of lactate was less obvious. Supplementation with lactate decreased cell proliferation and cell number. However, from the data obtained, we hypothesize that the surviving cells were able to adapt to the change in environment and utilize lactate as an energy source. There was no significant effect of lactate on the pluripotency of the cells. (C) 2016 Elsevier B.V. All rights reserved.