CDK-mediated RNF4 phosphorylation regulates homologous recombination in S-phase.
CDK-mediated RNF4 phosphorylation regulates homologous recombination in S-phase.
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CDK介导的RNF4磷酸化调节S期同源重组
DOI：
10.1093/nar/gkv434
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发表时间：
2015-06-23
影响因子：
14.9
通讯作者：
Lou Z
中科院分区：
文献类型：
--
作者：
Luo K;Deng M;Li Y;Wu C;Xu Z;Yuan J;Lou Z
DNA双链断裂的修复主要有两条途径：非同源末端连接（NHEJ）和同源重组（HR）。NHEJ在整个细胞周期中发挥作用，而HR主要在S/G2期发挥作用，提示存在细胞周期特异性机制调节NHEJ和HR之间的平衡。RNF 4磷酸化位点的突变导致MDC 1稳定，这反过来又损害了S期的HR。这些结果表明，除了驱动细胞周期进程外，CDK还靶向RNF 4，RNF 4参与DSB修复的调控网络。
There are the two major pathways responsible for the repair of DNA double-strand breaks (DSBs): non-homologous end-joining (NHEJ) and homologous recombination (HR). NHEJ operates throughout the cell-cycle, while HR is primarily active in the S/G2 phases suggesting that there are cell cycle-specific mechanisms that regulate the balance between NHEJ and HR. Here we reported that CDK2 could phosphorylate RNF4 on T26 and T112 and enhance RNF4 E3 ligase activity, which is important for MDC1 degradation and proper HR repair during S phase. Mutation of the RNF4 phosphorylation sites results in MDC1 stabilization, which in turn compromised HR during S-phase. These results suggest that in addition to drive cell cycle progression, CDK also targets RNF4, which is involved in the regulatory network of DSBs repair.
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影响因子：
11.4
作者：
Constantinou, A;Chen, XB;West, SC
通讯作者：
West, SC
影响因子：
10.5
作者：
Nimonkar, Amitabh V.;Genschel, Jochen;Kowalczykowski, Stephen C.
通讯作者：
Kowalczykowski, Stephen C.
影响因子：
7.7
作者：
Groocock, Lynda M.;Nie, Minghua;Boddy, Michael N.
通讯作者：
Boddy, Michael N.
人CTIP介导DNA终端切除和双链断裂修复的细胞周期控制。
DOI：
10.1074/jbc.m808906200
发表时间：
2009-04-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Huertas P;Jackson SP
通讯作者：
Jackson SP
影响因子：
10.5
作者：
Galanty, Yaron;Belotserkovskaya, Rimma;Jackson, Stephen P.
通讯作者：
Jackson, Stephen P.