Involvement of reactive oxygen species in 2-methoxyestradiol-induced apoptosis in human neuroblastoma cells.
Involvement of reactive oxygen species in 2-methoxyestradiol-induced apoptosis in human neuroblastoma cells.
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DOI：
10.1016/j.canlet.2011.09.005
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发表时间：
2011-12-27
期刊：
影响因子：
9.7
通讯作者：
Chen, Shao-yu
中科院分区：
文献类型：
--
作者：
Zhang, Qi;Ma, Yan;Cheng, Yue-Fang;Li, Wen-Jie;Zhang, Zhenzhong;Chen, Shao-yu
神经母细胞瘤是儿童最常见的颅外实体瘤。尽管儿童癌症的治疗取得了进展，但晚期神经母细胞瘤儿童的预后仍然很差。本文报道了2-甲氧基雌二醇(2-ME)对人神经母细胞瘤SK-N-SH和SH-SY5Y细胞的增殖抑制和诱导凋亡作用。2-ME还导致SK-N-SH和SH-SY5Y细胞产生ROS，线粒体膜电位丧失，提示2-ME诱导的细胞凋亡是由ROS介导的。结果表明，抗氧化剂Vc、L-GSH和MitoQ10共同处理SK-N-SH和SH-SY5Y细胞，可减少2-ME诱导的ROS的产生和线粒体膜电位的丧失，增加Bcl2/Bax比值，降低2-ME诱导的caspase-9和caspase-3的激活和凋亡诱导因子的上调，并阻止2-ME诱导的SK-N-SH和SH-SY5Y细胞的凋亡。这些结果提示，氧化应激在2-ME诱导人神经母细胞瘤细胞凋亡中起重要作用。
Neuroblastoma is the most common extra-cranial solid tumor in children. Despite advances in the treatment of childhood cancer, outcomes for children with advanced-stage neuroblastoma remain poor. Here we reported that 2-methoxyestradiol (2-ME) inhibited the proliferation and induced apoptosis in human neuroblastoma SK-N-SH and SH-SY5Y cells. 2-ME treatment also resulted in the generation of ROS and the loss of mitochondrial membrane potential in SK-N-SH and SH-SY5Y, indicating that 2-ME-induced apoptosis is mediated by ROS. This is supported by the results that have shown that co-treatment with antioxidants, VC, L-GSH and MitoQ10, decreased 2-ME-induced generation of ROS and the loss of the mitochondrial membrane potential, increased the Bcl-2/Bax ratio, decreased 2-ME-induced activation of caspase-9 and caspase-3 and the up-regulation of apoptosis-inducing factor (AIF), and prevented 2-ME-induced apoptosis in SK-N-SH and SH-SY5Y cells. These results suggested that oxidative stress plays an important role in 2-ME-induced apoptotic death of human neuroblastoma cells.
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影响因子：
5.3
作者：
Benhar, M;Dalyot, I;Levitzki, A
通讯作者：
Levitzki, A
影响因子：
4.8
作者：
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影响因子：
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作者：
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DOI：
10.1006/bbrc.1998.8870
发表时间：
1998-06-29
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
56.9
作者：
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通讯作者：
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