The prevention of peritendinous adhesions by a phospholipid polymer hydrogel formed in situ by spontaneous intermolecular interactions
The prevention of peritendinous adhesions by a phospholipid polymer hydrogel formed in situ by spontaneous intermolecular interactions
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DOI：
10.1016/j.biomaterials.2010.01.100
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Kawaguchi, Hiroshi
中科院分区：
文献类型：
--
作者：
Ishiyama, Noriyuki;Moro, Toru;Kawaguchi, Hiroshi
预防肌腱手术修复后的腱周粘连是困难的。为了建立一种理想的防粘连材料，我们通过在Fe 3+存在下混合两种聚合物（聚（MPC-co-甲基丙烯酸）（PMA）和两亲性聚（MPC-co-甲基丙烯酸正丁酯）（PMB））的水溶液来制备自发形成的水凝胶。这种PMA/PMB/Fe 3+水凝胶（MPC聚合物水凝胶）具有具有纳米级孔的蜂窝状微观结构，其抵抗细胞侵袭，但允许细胞因子和生长因子通过以用于肌腱愈合。水凝胶的解离速率可以通过改变Fe 3+浓度来控制，并且通过检查水凝胶的粘弹性，我们确定最佳Fe 3+浓度为0.05 M。然后，我们研究了原位应用的MPC聚合物水凝胶含有0.05 M的Fe 3+通过使用两种动物模型：大鼠跟腱模型和鸡屈趾深肌腱模型的影响。在两种模型中，宏观和组织学观察显示，水凝胶应用显著减少了腱周粘连。力学分析表明，水凝胶防止肌腱周围粘连，但不影响肌腱愈合。由于其特有的微结构和良好的生物相容性，我们认为MPC聚合物水凝胶将是预防腱周粘连的理想材料。(C)2010爱思唯尔有限公司保留所有权利。
Preventing peritendinous adhesions after surgical repair of tendon is difficult. In order to establish an ideal anti-adhesion material, we prepared a spontaneously forming hydrogel by mixing the aqueous solutions of two polymers, poly(MPC-co-methacrylic acid) (PMA) and amphiphilic poly(MPC-co-n-butyl methacrylate) (PMB), in the presence of Fe3+. This PMA/PMB/Fe3+ hydrogel (MPC polymer hydrogel) had a honeycomb microstructure with nanometer-scale pores, which resist cell invasion but allow the passage of cytokines and growth factors for tendon healing. The dissociation rate of the hydrogel could be controlled by changing Fe3+ concentration, and by examining the viscoelasticity of the hydrogel, we determined the optimal Fe3+ concentration to be 0.05 M. We then examined the effects of the in situ application of this MPC polymer hydrogel containing 0.05 M Fe3+ by using two animal models: the rat Achilles tendon model and the chicken flexor digitorum profundus tendon model. In both models, macroscopic and histological observation revealed that peritendinous adhesions were significantly decreased by the hydrogel application. Mechanical analyses revealed that the hydrogel prevented peritendinous adhesions but did not affect the tendon healing. Because of its characteristic microstructure and excellent biocompatibility, we believe that the MPC polymer hydrogel will be ideal for preventing peritendinous adhesions. (C) 2010 Elsevier Ltd. All rights reserved.