Nitratireductor lucknowense sp nov., a novel bacterium isolated from a pesticide contaminated soil
Nitratireductor lucknowense sp nov., a novel bacterium isolated from a pesticide contaminated soil
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DOI：
10.1007/s10482-011-9623-9
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发表时间：
2012-01-01
影响因子：
2.6
通讯作者：
Mayilraj, Shanmugam
中科院分区：
文献类型：
--
作者：
Manickam, Natesan;Pareek, Siddhika;Mayilraj, Shanmugam
从某农药污染场地分离到一株淡黄色细菌IITR-21（T），并对其进行了多相分类学研究。该生物的形态和化学分类学特征与其在Nitratireductor属中的分类一致。16 S rRNA基因序列分析表明，菌株IITR-21（T）属于Nitratireductor属，与Nitratireductor indicus C115（T）的亲缘关系较近。（97.7%）和太平洋硝化还原菌pht-3B（T）（97.4%），而与其它种包括模式种Nitratireductor，硝酸还原菌aquibiodomus的相似性低于97.0%。而菌株IITR-21（T）与中度相关的N. indicus（59.1%）和N. pacificus（40.4%）远低于70%的细菌物种划分阈值建议。DNA的G+C含量为62.4mol%。通过对菌株IITR-21（T）的生理生化特性及与已发表的硝酸还原菌属其它两个种的基因型差异的比较，将其分类为硝酸还原菌属的一个新种：勒克瑙硝酸还原菌（Nitratireductor lucknowense sp. nov.），模式菌株为IITR-21（T）（=MTCC 8354（T）= DSM 24322（T））。
A straw-yellow pigmented bacterium, strain IITR-21(T) was isolated from a pesticide contaminated site and characterized by using a polyphasic taxonomic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Nitratireductor. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain IITR-21(T) belongs to the genus Nitratireductor and was moderately related to Nitratireductor indicus C115(T) (97.7%) and Nitratireductor pacificus pht-3B(T) (97.4%), whereas sequence similarity value with the other species including the type species of the genus Nitratireductor, Nitratireductor aquibiodomus showed less than 97.0% similarity. However, the DNA-DNA relatedness values between strain IITR-21(T) and the moderately related taxa N. indicus (59.1%) and N. pacificus (40.4%) were well below the 70% threshold value recommended for the delineation of bacterial species. The G+C content of the DNA was 62.4 mol%. Based on physiological, biochemical tests and genotypic differences between the strain IITR-21(T) and the other two validly published species of the genus Nitratireductor, it is proposed that the strain be classified as a new species of Nitratireductor as Nitratireductor lucknowense sp. nov. The type strain is IITR-21(T) (=MTCC 8354(T) = DSM 24322(T)).