A proteomic approach to iron and copper homeostasis in cyanobacteria
A proteomic approach to iron and copper homeostasis in cyanobacteria
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DOI：
10.1093/bfgp/elm030
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发表时间：
2007-12-01
期刊：
影响因子：
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通讯作者：
De la Rosa, Miguel A.
中科院分区：
文献类型：
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作者：
De la Cerda, Berta;Castielli, Ornella;De la Rosa, Miguel A.
蓝藻被认为是叶绿体前体，是全球光合作用生产力的重要贡献者。含有不同金属（主要是铁和铜）的丰富种类的膜和可溶性蛋白质使这些生物体形成具有不同机制和严格调节过程的复杂稳态，以满足不断变化的环境中的金属需求。细胞代谢如此适应，以根据相对金属可用性合成替代蛋白质。特别是质体蓝素（一种铜蛋白）和细胞色素c（6）（一种血红素蛋白）可以相互替代，在光合作用和呼吸作用中发挥与电子载体相同的生理作用，其中一种或另一种蛋白质的合成受培养基中铜浓度的调节。单细胞蓝藻集胞藻属 sp。 PCC 6803由于其基因组序列完整且易于遗传操作而被广泛用作模型系统，并且正在进行大量蛋白质组学工作。在这篇综述文章中，我们重点关注质体蓝素和细胞色素 c(6) 敲除集胞藻突变体的功能表征，并讨论了在不同铜浓度下进行的正在进行的蛋白质组分析，以研究蓝藻金属稳态和细胞对环境条件变化的反应。
Cyanobacteria, which are considered to be the chloroplast precursors, are significant contributors to global photosynthetic productivity. The ample variety of membrane and soluble proteins containing different metals (mainly, iron and copper) has made these organisms develop a complex homeostasis with different mechanisms and tight regulation processes to fulfil their metal requirements in a changing environment. Cell metabolism is so adapted as to synthesize alternative proteins depending on the relative metal availabilities. In particular, plastocyanin, a copper protein, and cytochrome c(6), a haem protein, can replace each other to play the same physiological role as electron carriers in photosynthesis and respiration, with the synthesis of one protein or another being regulated by copper concentration in the medium. The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a model system because of completion of its genome sequence and the ease of its genetic manipulation, with a lot of proteomic work being done. In this review article, we focus on the functional characterization of knockout Synechocystis mutants for plastocyanin and cytochrome c(6), and discuss the ongoing proteomic analyses performed at varying copper concentrations to investigate the cyanobacterial metal homeostasis and cell response to changing environmental conditions.