Differentiating RNA from DNA by a molecular fluorescent probe based on the "door-bolt" mechanism biomaterials
Differentiating RNA from DNA by a molecular fluorescent probe based on the "door-bolt" mechanism biomaterials
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DOI：
10.1016/j.biomaterials.2018.05.050
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发表时间：
2018-09-01
期刊：
影响因子：
14
通讯作者：
Peng, Xiaojun
中科院分区：
文献类型：
--
作者：
Yao, Qichao;Li, Haidong;Peng, Xiaojun
尽管已经开发出了用于DNA的优良荧光探针，但是仍然缺乏用于RNA的优良探针。报道的和商业化的RNA探针的短缺归因于它们对DNA的严重干扰。由于DNA和RNA具有相似的结构和不同的功能，因此开发区别于DNA的RNA探针是一个迫切的挑战。在这项研究中，一个近红外荧光探针，NBE，被描述，它包含一个庞大的久洛尼定基团，可以适合在一个宽敞的RNA口袋，并发出强烈的荧光。然而，NBE对DNA没有反应，因为它不能插入双链，甚至不能插入DNA小沟。传感机制类似于门闩的效果。NBE在RNA传感方面表现出优异的性能（出色的光稳定性，高选择性和快速响应），无论是在水性缓冲液，固定细胞还是活细胞中。这些发现不仅提供了一种潜在的成像工具，而且还为识别RNA同时避免DNA干扰提供了一种新的设计策略。(C)2018爱思唯尔有限公司版权所有。
Although excellent florescent probes have been developed for DNA, good probes for RNA remain lacking. The shortage of reported and commercial RNA probes is attributable to their severe interference from DNA. As DNA and RNA have similar structures but different functions, it has been an imperative challenge to develop RNA probes that differentiate from DNA. In this study, an NIR fluorescent probe, NBE, is described, which contains a bulky julolidine group that can fit in a spacious RNA pocket and emit intense fluorescence. However, NBE has no response to DNA, as it cannot intercalate into the double strands or even in the DNA minor groove. The sensing mechanism is similar to the effect of a door-bolt. NBE shows excellent performance in RNA sensing (outstanding photostability, high selectivity and fast response), whether in aqueous buffers, fixed cells or living cells. These findings might provide not only a potential imaging tool but also a new design strategy for the recognition of RNA while avoiding interference from DNA. (C) 2018 Elsevier Ltd. All rights reserved.