THE EFFECT OF PARTICLE-SIZE ON THE OSTEOGENIC ACTIVITY OF COMPOSITE GRAFTS OF ALLOGENEIC FREEZE-DRIED BONE AND AUTOGENOUS MARROW
THE EFFECT OF PARTICLE-SIZE ON THE OSTEOGENIC ACTIVITY OF COMPOSITE GRAFTS OF ALLOGENEIC FREEZE-DRIED BONE AND AUTOGENOUS MARROW
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DOI：
10.1902/jop.1980.51.11.625
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发表时间：
1980-01-01
影响因子：
4.3
通讯作者：
YUKNA, A
中科院分区：
文献类型：
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作者：
SHAPOFF, CA;BOWERS, GM;YUKNA, A
本研究旨在确定粒度是否是评价冻干同种异体骨（FDBA）成骨活性的一个考虑因素，如果是，则确定小颗粒是增强还是抑制骨生成。将小颗粒FDBA（100-300 μ m）加骨髓和大颗粒FDBA（1000-2000 μ m）加骨髓置于固定于6只恒河猴股骨的有机玻璃扩散室中。对照室仅含有骨髓或留空。在第5周和第7周分别对两只动物注射盐酸土霉素，以获得活体骨标记。8周后取出所有小室。通过荧光显微镜评价10个腔室的新骨形成。另外15个腔的内容物通过单带技术评价是否存在骨吸收和骨化。与大颗粒FDBA（1000-2000 μ m）加自体骨髓相比，与小颗粒FDBA（100-300 μ m）加自体骨髓相关的新骨形成明显显著更多。小颗粒FDBA（100-300 μ m）加自体骨髓倾向于显示比大颗粒FDBA（1000-2000 μ m）加自体骨髓更多的再吸收。FDBA的小颗粒明显增强骨生成。当比较冻干同种异体骨的成骨潜力时，颗粒大小被证明是一个需要考虑的变量。
This study was carried out to determine if particle size is a factor to be considered in the evaluation of the osteogenic activity of freeze-dried bone allografts (FDBA) and, if so, whether small particles enhance or inhibit osteogenesis. Small particle FDBA (100-300 .mu.m) plus marrow and large particle FDBA (1000-2000 .mu.m) plus marrow were placed in plexiglass diffusion chambers secured to the femurs of 6 Rhesus monkeys. Control chambers contained marrow alone or were left empty. Two animals were given injections of oxytetracycline hydrochloride at 5 and 7 wk to obtain intravital osseous labeling. All chambers were removed after 8 wk. Ten chambers were evaluated for new bone formation by fluorescent microscopy. The contents of 15 additional chambers were evaluated by single band technique for presence or absence of bone resorption and ossification. There was apparently, significantly more new bone formation associated with small particle FDBA (100-300 .mu.m) plus autogenous marrow than with large particle FDBA (1000-2000 .mu.m) plus autogenous marrow. Small particle FDBA (100-300 .mu.m) plus autogenous marrow tended to display more resorption than large particle FDBA (1000-2000 .mu.m) plus autogenous marrow. Small particles of FDBA evidently enhance osteogenesis. Particle size was demonstrated to be a variable to be considered when comparing the osteogenic potential of freeze-dried bone allografts.