DISCREPANCY BETWEEN PENNER SEROTYPING AND POLYMERASE CHAIN-REACTION FINGERPRINTING OF CAMPYLOBACTER ISOLATED FROM POULTRY AND OTHER ANIMAL SOURCES
DISCREPANCY BETWEEN PENNER SEROTYPING AND POLYMERASE CHAIN-REACTION FINGERPRINTING OF CAMPYLOBACTER ISOLATED FROM POULTRY AND OTHER ANIMAL SOURCES
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DOI：
10.1111/j.1472-765x.1995.tb01324.x
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发表时间：
1995-06-01
影响因子：
2.4
通讯作者：
JACOBSREITSMA, WF
中科院分区：
文献类型：
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作者：
AARTS, HJM;VANLITH, LAJT;JACOBSREITSMA, WF
从荷兰两个肉鸡养殖场不同生产周期的各种家畜和暗色甲虫中分离出34株空肠弯曲菌或大肠埃希氏菌，进行了Penner血清分型和聚合酶链式反应(PCR)指纹分析。在分离的菌株中确定了10种不同的Penner血清型。聚合酶链式反应指纹的视觉评分产生了14个明显不同的特征。部分Penner血清型相同的菌株表现出不同的PCR指纹图谱，而不同血清型的菌株表现出相同的PCR指纹图谱。来自不同动物来源的分离株之间Penner血清分型和PCR指纹图谱的差异最为明显。发现了发生基因组重排的迹象。血清分型和弯曲菌指纹图谱之间的不一致表明，如果要可靠地评估导致活鸡弯曲菌定植的流行病学因素，就应该使用传统的分型方法与指纹分析相结合。
Thirty-four Campylobacter jejuni or coli strains, isolated from various livestock and darkling beetles from two Dutch poultry farms during different broiler production cycles, were subjected to Penner serotyping and polymerase chain reaction (PCR) fingerprint analysis. Ten different Penner serotypes were determined in the isolates. Visual scoring of the PCR fingerprints resulted in 14 clearly different profiles. Some strains with identical Penner serotypes exhibited different PCR fingerprints and conversely strains with different serotypes produced identical PCR fingerprints. Discrepancies between Penner serotyping and PCR fingerprinting were most obvious between isolates from different animal sources. Indications for the occurrence of genomic rearrangements were found. The inconsistency between serotyping and fingerprinting of Campylobacter strains suggests that conventional typing methods should be used in combination with fingerprinting if the epidemiological factors that contribute to Campylobacter colonization of live chickens are to be assessed reliably.