Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation
Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation
复制标题
使用具有膜到核易位的分子转子探针探测细胞膜损伤
DOI：
10.1039/d0mh01141j
复制
发表时间：
2020-12-01
影响因子：
13.3
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Wang, Kang-Nan;Qi, Guobin;Liu, Bin
细胞膜（最外层的保护层）的损坏对细胞来说是致命的。然而，精确监测和原位报告细胞膜损伤并非易事。在此，我们提出了一种分子转子探针TPAE2，它可以有效地结合溶液中的DNA和1,2-二油酰-sn-甘油-3-磷酸胆碱。由于分子转子的发光成像特性，TPAE2 可以对质膜进行超快速、免洗染色，具有 160 倍的荧光“开启”和出色的光稳定性。一旦膜受损，TPAE2 就可以作为信号报告基因点亮细胞核。利用TPAE2对细胞膜和细胞核进行级联成像，能够实时跟踪细胞凋亡的全过程。更重要的是，在照射下，染色在细胞膜上的TPAE2可以快速穿透细胞并选择性地染色细胞核，自我报告癌细胞消融过程。这是具有多种功能的单个分子可以点亮细胞核作为细胞膜损伤指示的第一个例子。膜到核的易位策略为生物医学应用的膜损伤诊断探针的设计开辟了一条新途径。
Damage to cell membranes, the outermost protection layer, is fatal to cells. However, precisely monitoring and in situ reporting cell membrane damage is not trivial. Herein, we present a molecular rotor probe, TPAE2, which can effectively bind to DNA and 1,2-dioleoyl-sn-glycero-3-phosphocholine in solution. Due to the light-up imaging characteristics of the molecular rotor, TPAE2 offers ultrafast and wash-free staining of plasma membrane with 160-fold fluorescence "turn-on" and excellent photostability. Once the membrane is damaged, TPAE2 can light-up the nucleus as a signal reporter. The cascade imaging of the cell membrane and nucleus using TPAE2 enabled real-time tracking of the whole process of cell apoptosis. What's more, under irradiation, TPAE2 stained on the cell membrane could penetrate cells rapidly and selectively stain the nucleus, self-reporting the cancer cell ablation process. This is the first example that a single molecule with multiple functions can light up the nucleus as an indication of cell membrane damage. The membrane-to-nucleus translocation strategy opens up a new avenue for the design of membrane damage diagnosis probes for biomedical applications.